MERCK 110582 Sodium chloride peptone broth (buffered) acc. EP, USP, JP GranuCult^®
Catalogue Number	110582
Overview	GranuCult™ is our new brand name for our existing granulated dehydrated culture media which apply to new EN ISO 11133:2014 and various other ISO standards and FDA-BAM / USDA-FSIS methods for testing of food and water samples. All these media are currently being updated to be fully compliant to the latest version of these standards and regulations with respect to composition and quality control and will be available shortly.

MERCK 110582 Sodium chloride peptone broth (buffered) acc. EP, USP, JP GranuCult^®

Catalogue Number

110582

Overview

GranuCult™ is our new brand name for our existing granulated dehydrated culture media which apply to new EN ISO 11133:2014 and various other ISO standards and FDA-BAM / USDA-FSIS methods for testing of food and water samples. All these media are currently being updated to be fully compliant to the latest version of these standards and regulations with respect to composition and quality control and will be available shortly.

Product Information
HS Code	3821 00 00
Quality Level	MQ100

Physicochemical Information
pH value	6.8 - 7.2 (16.1 g/l, H₂O, 25 °C) (after autoclaving)
Solubility	16.1 g/l

Safety Information according to GHS
Storage class	10 - 13 Other liquids and solids
WGK	WGK 1 slightly hazardous to water
Disposal	14 Inorganic salts: Container I. Neutral solutions of the these salts: Container D. Before placing in Container D, check the pH with pH-Universal indicator strips (Item No. 109535).

Storage and Shipping Information
Storage	Store at +15 °C to +25 °C.

Specifications
Appearance (clearness)	clear
Appearance (color)	slightly yellowish to yellowish
pH-value (25 °C)	6.8 - 7.2
Typical composition (g/litre): Potassium dihydrogen phosphate 3.6; di-Sodium hydrogen phosphate dihydrate 7.2; Sodium chloride 4.3; Peptone from meat 1.0.
Growth promotion test in accordance with the current version of DIN EN ISO 11133 and harmonised method of EP, USP and JP.
Inoculum on reference medium (Escherichia coli ATCC 8739 (WDCM 00012))	≤ 100
Inoculum on reference medium (Escherichia coli ATCC 25922 (WDCM 00013))	≤ 100
Inoculum on reference medium (Staphylococcus aureus ATCC 6538 (WDCM 00032))	≤ 100
Inoculum on reference medium (Staphylococcus aureus ATCC 25923 (WDCM 00034))	≤ 100
Colony count (Escherichia coli ATCC 8739 (WDCM 00012))
Colony count (Escherichia coli ATCC 25922 (WDCM 00013))
Colony count (Staphylococcus aureus ATCC 6538 (WDCM 00032))
Colony count (Staphylococcus aureus ATCC 25923 (WDCM 00034))
Recovery (Escherichia coli ATCC 8739 (WDCM 00012))	70 - 130 %
Recovery (Escherichia coli ATCC 25922 (WDCM 00013))	70 - 130 %
Recovery (Staphylococcus aureus ATCC 6538 (WDCM 00032))	70 - 130 %
Recovery (Staphylococcus aureus ATCC 25923 (WDCM00034))	70 - 130 %
Incubation: 45 min. at roomtemperature aerobic. Reference medium: Tryptic Soy Agar (24 hours at 35 - 37 °C aerobic).
The information above is current at this time of publication and is subject to change without notice (except for customers holding a change control agreement with our company). The information/format can be altered at any time and inany way if internal company matters or Standard Regulationsdo say so.

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