Sigma Aldrich A2942 Amphotericin B solution 250 μg/mL in deionized water, 0.1 μm filtered, BioReagent, suitable for cell culture 500 mL
Marka
Stok Kodu
LB.SA.A2942-500ML
Kısa Bilgi
Synonym(s): Amphotericin, Fungizone Empirical Formula (Hill Notation): C47H73NO17 CAS Number: 1397-89-3 Molecular Weight: 924.08 MDL number: MFCD00877763 PubChem Substance ID: 24890711 NACRES: NA.76
| Sigma Aldrich A2942 Amphotericin B solution |
|
Synonym(s): Amphotericin, Fungizone
Empirical Formula (Hill Notation): C47H73NO17
CAS Number: 1397-89-3
Molecular Weight: 924.08
MDL number: MFCD00877763
PubChem Substance ID: 24890711
NACRES: NA.76
|
|
PROPERTIES
Quality Level 200
sterility 0.1 μm filtered
product line BioReagent
form liquid
concentration 250 μg/mL in deionized water
technique(s) cell culture | mammalian: suitable
color yellow antibiotic activity spectrum fungi yeast
Mode of action cell membrane | interferes
shipped in dry ice
storage temp. −20°C
SMILES string [H][C@]12C[C@@H](O[C@@H]3O[C@H](C)[C@@H](O)[C@H](N)[C@@H]3O)\C=C\C=C\C=C\C=C\C=C\C=C\C=C\[C@H](C)[C@@H](O)[C@@H](C)[C@H](C)OC(=O)C[C@H](O)C[C@H](O)CC[C@@H](O)[C@H](O)C[C@H](O)C[C@](O)(C[C@H](O)[C@H]1C(=O)O[Na])O2
InChI 1S/C47H73NO17/c1-27-17-15-13-11-9-7-5-6-8-10-12-14-16-18-34(64-46-44(58)41(48)43(57)30(4)63-46)24-38-40(45(59)60)37(54)26-47(61,65-38)25-33(51)22-36(53)35(52)20-19-31(49)21-32(50)23-39(55)62-29(3)28(2)42(27)56/h5-18,27-38,40-44,46,49-54,56-58,61H,19-26,48H2,1-4H3,(H,59,60)/b6-5+,9-7+,10-8+,13-11+,14-12+,17-15+,18-16+/t27-,28-,29-,30+,31+,32+,33-,34-,35+,36+,37-,38-,40+,41-,42+,43+,44-,46-,47+/m0/s1
InChI key APKFDSVGJQXUKY-INPOYWNPSA-N
DESCRIPTION
General description
Amphotericin B is a macrocyclic lactone with a hydrophilic polyhydroxyl chain and a lipophilic polyene based hydrocarbon chain.[1]
Application
Amphotericin B solution has been used: as an intraperitoneal injection for the treatment of Aspergillus fumigatus infection in mice[2] as a constituent of aspiration medium for the culture of Chinese Merino ewe ovaries as a constituent of transport medium during the collection of equine corpora lutea[3] as a supplement in RPMI medium for the culture of the mouse-mouse B cell hybridoma 55-6 cell line[4]
Biochem/physiol Actions
Amphotericin B is a polyene antifungal antibiotic from Streptomyces sp. that is clinically used to treat systemic fungal infections.[5] It has a high affinity for sterols, especially ergosterols, of both fungal and bacterial cell membranes. Normal usage in maintenance of antifungal cell cultures is 2.5 mg/L with penicillin and streptomycin used in the medium. Amphotericin B is most effective against fungi and yeast.[6] It forms transmembrane channels by binding to sterols associated with membranes, thereby leading to the leakage of small molecules.[6] Amphotericin B elicits toxicity despite being an effective antibiotic for treating mycoses.[7]
Packaging
20,50,100ml
Components
This product is supplied as 250 μg/mL Amphotericin B in deionized water. It is a solution containing Amphotericin B, and the following components aimed to improve its solubility: sodium deoxycholate, sodium chloride and sodium phosphate. Caution
Amphotericin B remains active for 3 days in culture at 37°C.
Preparation Note
It is not unusual for this material to form a precipitate in aqueous solutions. Mixing the solution will redissolve most of the precipitate but, if it does not fully resolubilize, the Amphotericin B will still be suitable for use.
Other Notes
Keep container tightly closed in a dry and well-ventilated place. Light sensitive.
|
