Sigma-Aldrich D0632 DL-Dithiothreitol ≥98% (HPLC), ≥99.0% (titration) 25 gr
| Sigma-Aldrich D0632 DL-Dithiothreitol ≥98% (HPLC), ≥99.0% (titration) 25 gr |
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Synonym(s): threo-1,4-Dimercapto-2,3-butanediol, Cleland’s reagent, DTT
Linear Formula: HSCH2CH(OH)CH(OH)CH2SH
CAS Number: 3483-12-3
Molecular Weight: 154.25
Beilstein: 1719757
EC Number: 222-468-7
MDL number: MFCD00004877
PubChem Substance ID: 24893251
NACRES: NA.21
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PROPERTIES
Quality Level 200
assay ≥98% (HPLC), ≥99.0% (titration)
form powder
reaction suitability reagent type: reductant
color white
mp 41-44 °C (lit.)
solubility H2O: soluble 50 mg/mL, clear, colorless to very faintly yellow
storage temp. 2-8°C
SMILES string O[C@H](CS)[C@H](O)CS
InChI 1S/C4H10O2S2/c5-3(1-7)4(6)2-8/h3-8H,1-2H2/t3-,4-/m1/s1
InChI key VHJLVAABSRFDPM-QWWZWVQMSA-N
DESCRIPTION
General description
An excellent reagent for maintaining SH groups in reduced state; quantitatively reduces disulfides. DTT is effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE. DTT can also be used for reducing the disulfide bridge of the cross-linker N,N′-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel. DTT is less pungent and is less toxic than 2-mercaptoethanol. Typically, a seven fold lower concentration of DTT (100 mM) is needed than is used for 2-mercaptoethanol (5% v/v, 700 mM).
Application
DTT has been used:
as one of the reactants in the reduction and alkylation of αs1-Casein, the major allergen of cow′s milk.
as a component of medium for the demembranation and reactivation of spermatozoa.
to maintain stability of the enzyme as thiol effectively protects the active sites of the biocatalyst.
as a reducing agent to test the specificity of the reaction of N-Ethylmaleimide with sulfhydryl groups.
An excellent reagent for maintaining SH groups in reduced state; quantitatively reduces disulfides. DTT is effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE. DTT can also be used for reducing the disulfide bridge of the cross-linker N,N′-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel. DTT is less pungent and is less toxic than 2-mercaptoethanol. Typically, a seven fold lower concentration of DTT (100 mM) is needed than is used for 2-mercaptoethanol (5% v/v, 700 mM).
Packaging
1, 5, 10, 25, 100 g in poly bottle
1 kg in poly drum |