Sigma-Aldrich P1379 TWEEN 20 viscous liquid 250 mL
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Sigma-Aldrich P1379 TWEEN® 20 viscous liquid 250 mL |
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Synonym(s): Polyethylene glycol sorbitan monolaurate, Polyoxyethylenesorbitan monolaurate
CAS Number: 9005-64-5
MDL number: MFCD00165986
NACRES: NA.21
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PROPERTIES
description non-ionic
Quality Level 300
assay ≥40.0% (GC)
form viscous liquid
mol wt ~1228
composition lauric acid, ≥40% (balance primarily myristic, palmitic, and stearic acids)
technique(s)
DNA extraction: suitable
RNA extraction: suitable
cell culture | mammalian: suitable
protein purification: suitable
impurities ≤3.0% water
refractive index n20/D 1.468 (lit.)
CMC 0.06 mM (20-25°C)
acid number ≤2.2 mg/g
hydroxyl value 96‑108 mg/g
transition temp cloud point 76 °C
density 1.095 g/mL at 25 °C (lit.)
HLB 16.7
suitability complies for IR spectroscopy
InChI 1S/C26H50O10/c1-2-3-4-5-6-7-8-9-10-11-24(30)34-19-18-31-20-22(32-15-12-27)26-25(35-17-14-29)23(21-36-26)33-16-13-28/h22-23,25-29H,2-21H2,1H3
InChI key HMFKFHLTUCJZJO-UHFFFAOYSA-N
DESCRIPTION
General description
Tween®20 is a polyoxyethylene sorbitol ester that belongs to the polysorbate family. It is a nonionic detergent having a molecular weight of 1,225 daltons, assuming 20 ethylene oxide units, 1 sorbitol, and 1 lauric acid as the primary fatty acid. The ethylene oxide subunits are responsible for the hydrophilic nature of the surfactant, while the hydrocarbon chains provide the hydrophobic environment. Sorbitol forms the backbone ring to which the ethylene oxide polymers are attached.
Application
TWEEN 20 has been used as a component of:
washing buffer and blocking buffer in Western
blotting blocking buffer in immunohistochemistry washing
buffer in ELISA (enzyme linked immunosorbent assay)
reaction mixture in PCR (polymerase chain reaction) TWEEN 20 is a nonionic detergent widely used in biochemical applications. It has been used as an emulsifying agent for the preparation of stable oil-in-water emulsions. TWEEN 20 has been used in pre-extraction of membranes to remove peripheral proteins (used at 2% for extraction of membrane-bound proteins). TWEEN 20 has been used as a blocking agent for membrane based immunoassays at a typical concentration of 0.05%. TWEEN 20 can be used for lysing mammalian cells at a concentration of 0.005 to 0.5%. Non-ionic detergent
Packaging
25, 100, 250, 500, 6×500 mL in poly bottle
1, 4 L in poly bottle
P1379-1KT: Each kit contains 3 x 100ML samples, each sample from a uniquely manufactured lot.
Preparation Note
TWEEN 20 is miscible in water (100mg/mL), yielding a clear, yellow solution. It is also miscible with alcohol, dioxane, and ethyl acetate. TWEEN 20 is insoluble in liquid paraffin and fixed oils.
Legal Information
TWEEN is a registered trademark of Croda International PLC |