Sigma-Aldrich PPC202 ReadyShield® Protease and Phosphatase Inhibitor Cocktail non-freezing solution 5 mL
Marka
Stok Kodu
LB.SA.PPC2020-5ML
Kısa Bilgi
Synonym(s): Protease and phosphatase inhibitor cocktail, inhibitor cocktail
| Sigma-Aldrich PPC202 ReadyShield® Protease and Phosphatase Inhibitor Cocktail 5 mL |
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Synonym(s): Protease and phosphatase inhibitor cocktail, inhibitor cocktail
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PROPERTIES
Quality Level 200
form ready-to-use solution
packaging pkg of 1 mL (in amber polypropylene vial), pkg of 5 mL (in amber polypropylene vial)
storage temp. −20°C
DESCRIPTION
General description
Protease and Phosphatase Inhibitor Cocktail has been optimized and tested for mammalian cellular and tissue extracts. It contains inhibitors with a broad specificity for serine, cysteine, acid proteases, aminopeptidases, L-isozymes of alkaline phosphatases, and serine/ threonine protein phosphatases.
Protease and Phosphatase Inhibitor Cocktail has been tested on extracts from various animal tissues such as human placenta and bovine liver, and on extracts of A431, CHO, and U937 cells.
The cocktail is supplied as a ready-to-use solution using a proprietary non-freezing formulation. ReadyShield® PPC2020 is a non-freezing formulation that contains the same inhibitors as is the DMSO based cocktail PPC1010
Features and Benefits
Does not freeze in storage for quick, convenient use.
For broad ranging inhibition of both proteases and phosphatases.
Suggested as 100 X solution.
Reclosable screw-top vials.
Can be stored in cooler for approx. 1 month or room temperature for a week.
Preparation Note
The recommended dilution of the cocktail in the biological extract is 1 ml of the cocktail per 500 mg of protein extracted from tissue or cells. In many cases, the cocktail can be used at a final concentration of 1% (v/v), 1 ml of cocktail solution per 100 ml of extraction buffer.
Other Notes
For life science research only. Not for use in diagnostic procedures.
Legal Information
ReadyShield is a registered trademark of Merck KGaA, Darmstadt, Germany |
