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ZYMO RESEARCH E2018 dsDNA Shearase Plus 500 U
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ZYMO RESEARCH E2018 dsDNA Shearase Plus 500 U

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LB.MB.ZYMD5218-50
ZYMO RESEARCH E2018 dsDNA Shearase Plus 50 U
The simplest method for generating random-ended dsDNA fragments.
Fragment size can be controlled by adjusting the enzyme concentration.
Fragments generated using dsDNA Shearase Plus are ideal for library construction, Next-Gen sequencing, methylated DNA immunopreciptation (MeDIP, MeDIP-Seq), etc.
ZYMO RESEARCH E2018 dsDNA Shearase Plus 50 U 
dsDNA Shearase Plus is an endonuclease that cleaves phosphodiester bonds in DNA to yield oligonucleotides with 5’-phosphate and 3’-hydroxyl termini. It has a particularly strong preference for dsDNA and generates random-ended DNA fragments of the desired size in a single step. This enzyme is compatible with low volume inputs, thus minimizing sample loss.
Concentration 1 U/µl
Enzyme Inactivation Heat inactivate enzyme at 65°C for 5 min.
Storage Store at -20°C for up to 12 months. Avoid repeated freeze/thawing of reagents. Prolonged storage is at ≤ -70°C.
Unit Definition One unit (1 U) is defined at the amount of enzyme required to convert 250 ng human DNA into DNA fragments in the range of 100-500 bp in 20 minutes at 42°C in total reaction volume in 10 µl.
 ZYMO RESEARCH ürünleri labor.com.tr'deZYMO RESEARCH E2018 dsDNA Shearase Plus 50 U
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ZYMO RESEARCH E2018 dsDNA Shearase Plus 500 U

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