| Appearance (clearness) |
clear |
| Typical composition (per vial): D-Cycloserine 200 mg; 4-Methylumbelliferylphosphate di sodium salt 50 mg. |
Growth promotion test in accordance with ISO 11133.
Supplement tested with base Art. 1.11972. |
Inoculum on reference medium
- Clostridium perfringens ATCC 10543 |
10 - 100 |
| - Clostridium perfringens ATCC 13124 (WDCM 00007) |
10 - 100 |
| - Clostridium perfringens ATCC 12916 (WDCM 00080) |
10 - 100 |
| - Clostridium tetani ATCC 19406 |
> 10⁴ |
| - Clostridium novyi ATCC 17861 |
> 10⁴ |
| - Pseudomonas aeruginosa ATCC 27853 (WDCM 00025) |
> 10⁴ |
| - Bacillus cereus ATCC 11778 (WDCM 00001) |
> 10⁴ |
| - Escherichia coli ATCC 25922 (WDCM 00013) |
> 10⁴ |
| - Escherichia coli ATCC 8739 (WDCM 00012) |
> 10⁴ |
Recovery on test medium
- Clostridium perfringens ATCC 10543 |
≥ 70 % |
| - Clostridium perfringens ATCC 13124 (WDCM 00007) |
≥ 70 % |
| - Clostridium perfringens ATCC 12916 (WDCM 00080) |
≥ 70 % |
Growth
- Clostridium tetani ATCC 19406 |
none to fair |
| - Clostridium novyi ATCC 17861 |
none to fair |
| - Pseudomonas aeruginosa ATCC 27853 (WDCM 00025) |
none to poor |
| - Bacillus cereus ATCC 11778 (WDCM 00001) |
none to fair |
| - Escherichia coli ATCC 25922 (WDCM 00013) |
inhibited |
| - Escherichia coli ATCC 8739 (WDCM 00012) |
inhibited |
Blacking
- Clostridium perfringens ATCC 10543 |
+ |
| - Clostridium perfringens ATCC 13124 (WDCM 00007) |
+ |
| - Clostridium perfringens ATCC 12916 (WDCM 00080) |
+ |
| - Clostridium tetani ATCC 19406 |
- |
| - Clostridium novyi ATCC 17861 |
- |
| - Pseudomonas aeruginosa ATCC 27853 (WDCM 00025) |
- |
| - Bacillus cereus ATCC 11778 (WDCM 00001) |
- |
| - Escherichia coli ATCC 25922 (WDCM 00013) |
- |
| - Escherichia coli ATCC 8739 (WDCM 00012) |
- |
Fluorescence
- Clostridium perfringens ATCC 10543 |
+ |
| - Clostridium perfringens ATCC 13124 (WDCM 00007) |
+ |
| - Clostridium perfringens ATCC 12916 (WDCM 00080) |
+ |
Incubation: 20 hrs. 37 °C.
Reference: TSC-agar for l.perfingens; Blood-agar for all other strains. |
