MERCK 107700 RVS (RAPPAPORT-VASSILIADIS-Soya) broth (base) acc. ISO 6579 GranuCult® 500 gr
Catalogue Number	107700
Overview	GranuCult™ is our new brand name for our existing granulated dehydrated culture media which apply to new EN ISO 11133:2014 and various other ISO standards and FDA-BAM / USDA-FSIS methods for testing of food and water samples. All these media are currently being updated to be fully compliant to the latest version of these standards and regulations with respect to composition and quality control and will be available shortly.

MERCK 107700 RVS (RAPPAPORT-VASSILIADIS-Soya) broth (base) acc. ISO 6579 GranuCult® 500 gr

Catalogue Number

107700

Overview

GranuCult™ is our new brand name for our existing granulated dehydrated culture media which apply to new EN ISO 11133:2014 and various other ISO standards and FDA-BAM / USDA-FSIS methods for testing of food and water samples. All these media are currently being updated to be fully compliant to the latest version of these standards and regulations with respect to composition and quality control and will be available shortly.

Product Information
HS Code	3821 00 00
Quality Level	MQ100

Physicochemical Information
pH value	5.0 - 5.4 (42.5 g/l, H₂O, 25 °C) (after autoclaving)
Bulk density	700 kg/m3

Safety Information according to GHS
Storage class	10 - 13 Other liquids and solids
WGK	WGK 1 slightly hazardous to water

Storage and Shipping Information
Storage	Store at +15 °C to +25 °C.

Specifications
Appearance (clearness)	clear
Appearance (color)	darkblue
pH-value (25 °C)	5.0 - 5.4
Growth promotion test in accordance with the current version of DIN EN ISO 11133. Mixed inoculum of Salmonella, E.coli and P.aeruginosa Additionaly monoculture of E.coli and E.faecalis Confirmation on XLD agar resp. Tryptic soy agar
Inoculum on reference medium (Salmonella typhimurium ATCC 14028 (WDCM 00031))	≤ 100
Inoculum on reference medium (Salmonella enteritidis ATCC 13076 (WDCM 00030))	≤ 100
Inoculum on reference medium (Escherichia coli ATCC 25922 (WDCM 00013))	≥ 10000
Inoculum on reference medium (Escherichia coli ATCC 8739 (WDCM 00012))	≥ 10000
Inoculum on reference medium (Pseudomonas aeruginosa ATCC 27853 (WDCM 00025))	≥ 10000
Inoculum on reference medium (Enterococcus faecalis ATCC 29212 (WDCM 00087))	≥ 10000
Inoculum on reference medium (Enterococcus faecalis ATCC 19433 (WDCM 00009))	≥ 10000
Growth on XLD agar (Salmonella typhimurium ATCC 14028 (WDCM 00031))	> 10 colonies
Growth on XLD agar (Salmonella enteritidis ATCC 13076 (WDCM 00030))	> 10 colonies
Colonies (Salmonella typhimurium ATCC 14028 (WDCM 00031))	colourless with black centre
Colonies (Salmonella enteritidis ATCC 13076 (WDCM 00030))	colourless with black centre
Growth on Tryptic soy agar (Escherichia coli ATCC 25922 (WDCM 00013))	< 100 colonies
Growth on Tryptic soy agar (Escherichia coli ATCC 8739 (WDCM 00012))	< 100 colonies
Growth on Tryptic soy agar (Enterococcus faecalis ATCC 29212 (WDCM 00087))	< 10 colonies
Growth on Tryptic soy agar (Enterococcus faecalis ATCC 19433 (WDCM 00009))	< 10 colonies
Incubation: RVS broth: 24 ± 3 hours at 41.5 ± 1 °C aerobic Confirmation on XLD agar resp. Tryptic Soy Agar: 24 ± 3 hours at 37 ± 1 °C aerobic

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Merck 107700 Rvs (Rappaport-Vassılıadıs-Soya) Broth (Base) Acc. Iso 6579 granucult® 500 gr

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