| Appearance (clearness) |
clear |
| Appearance (colour) |
red |
| Solidification behaviour (4 hrs., 45 °C) |
liquid |
| pH-value (25 °C) |
7.2 - 7.6 |
| Typical composition (g/litre): Peptone from gelatine 7.0; Yeast extract 3.0; Sodium chloride 5.0; D(+)Glucose 10.0; Bile salt mixture 1.5; Neutralred 0.03; Crystal violet 0.002; Agar-agar 13.0. |
| Growth promotion test in accordance with the current version of harmonised method of EP, USP and JP. |
| Inoculum on reference medium (Escherichia coli ATCC 8739 (WDCM 00012)) |
10 - 100 |
| Inoculum on reference medium (Pseudomonas aeruginosa ATCC 9027 (WDCM 00026)) |
10 - 100 |
| Inoculum (Staphylococcus aureus ATCC 6538 (WDCM 00032)) |
≥ 10000 |
| Inoculum (Bacillus cereus ATCC 11778 (WDCM 00001)) |
≥ 10000 |
| Colony count (Escherichia coli ATCC 8739 (WDCM 00012)) |
|
| Colony count (Pseudomonas aeruginosa ATCC 9027 (WDCM 00026)) |
|
| Recovery on test medium (Escherichia coli ATCC 8739 (WDCM 00012)) |
50 - 150 % |
| Recovery on test medium (Pseudomonas aeruginosa ATCC 9027 (WDCM 00026)) |
50 - 150 % |
| Colony colour (Escherichia coli ATCC 8739 (WDCM 00012)) |
red colonies |
| Colony colour (Pseudomonas aeruginosa ATCC 9027 (WDCM 00026)) |
nearly colourless to slightly red colonies |
| Precipitate (Escherichia coli ATCC 8739 (WDCM 00012)) |
+ |
| Growth (Staphylococcus aureus ATCC 6538 (WDCM 00032)) |
no growth |
| Growth (Bacillus cereus ATCC 11778 (WDCM 00001)) |
no growth |
Incubation:
up to 18 hours at 30-35°C aerobic
Staphylococcus aureus and Bacillus cereus at least 24 hours.
Reference medium: Tryptic Soy Agar |
| Growth promotion test in accordance with the current version of DIN EN ISO 11133. |
| Inoculum on reference medium (Escherichia coli ATCC 8739 (WDCM 00012)) |
|
| Inoculum on reference medium (Escherichia coli ATCC 25922 (WDCM 00013)) |
|
| Inoculum on reference medium (Salmonella typhimurium ATCC 14028 (WDCM 00031)) |
|
| Inoculum on reference medium (Salmonella enteritidis ATCC 13076 (WDCM 00030)) |
|
| Colony count (Escherichia coli ATCC 8739 (WDCM 00012)) |
|
| Colony count (Escherichia coli ATCC 25922 (WDCM 00013)) |
|
| Colony count (Salmonella typhimurium ATCC 14028 (WDCM 00031)) |
|
| Colony count (Salmonella enteritidis ATCC 13076 (WDCM 00030)) |
|
| Recovery on test medium (Escherichia coli ATCC 8739 (WDCM 00012)) |
≥ 50 % |
| Recovery on test medium (Escherichia coli ATCC 25922 (WDCM 00013)) |
≥ 50 % |
| Recovery on test medium (Salmonella typhimurium ATCC 14028 (WDCM 00031)) |
≥ 50 % |
| Recovery on test medium (Salmonella enteritidis ATCC 13076 (WDCM 00030)) |
≥ 50 % |
| Colonies (Escherichia coli ATCC 8739 (WDCM 00012)) |
Pink to red colonies with or without precipitation halo |
| Colonies (Escherichia coli ATCC 25922 (WDCM 00013)) |
Pink to red colonies with or without precipitation halo |
| Colonies (Salmonella typhimurium ATCC 14028 (WDCM 00031)) |
Pink to red colonies with or without precipitation halo |
| Colonies (Salmonella enteritidis ATCC 13076 (WDCM 00030)) |
Pink to red colonies with or without precipitation halo |
| Growth (Enterococcus faecalis ATCC 29212 (WDCM 00087)) |
total inhibition |
| Growth (Enterococcus faecalis ATCC 19433 (WDCM 00009)) |
total inhibition |
Incubation: 24 ± 2 hours at 37 ± 1 °C
A recovery rate of 50 % is equivalent to a productivity value of 0.5.
The indicated colony counts result from the sum of a triple determination.
Reference medium: Tryptic Soy Agar |
| The information above is current at this time of publication and is subject to change without notice (except for customers holding a change control agreement with our company). The information/format can be altered at any time and inany way if internal company matters or Standard Regulationsdo say so. |
