MERCK 110660 MRS agar (de MAN, ROGOSA and SHARPE) acc. ISO 15214 GranuCult® 500 gr
Catalogue Number	110660
Overview	GranuCult™ is our new brand name for our existing granulated dehydrated culture media which apply to new EN ISO 11133:2014 and various other ISO standards and FDA-BAM / USDA-FSIS methods for testing of food and water samples. All these media are currently being updated to be fully compliant to the latest version of these standards and regulations with respect to composition and quality control and will be available shortly.

MERCK 110660 MRS agar (de MAN, ROGOSA and SHARPE) acc. ISO 15214 GranuCult® 500 gr

Catalogue Number

110660

Overview

GranuCult™ is our new brand name for our existing granulated dehydrated culture media which apply to new EN ISO 11133:2014 and various other ISO standards and FDA-BAM / USDA-FSIS methods for testing of food and water samples. All these media are currently being updated to be fully compliant to the latest version of these standards and regulations with respect to composition and quality control and will be available shortly.

Product Information
HS Code	3821 00 00
Quality Level	MQ300

Physicochemical Information
pH value	5.5 - 5.9 (66.24 g/l, H₂O, 25 °C) (after autoclaving)
Bulk density	600 kg/m3
Solubility	68.2 g/l

Safety Information according to GHS
Storage class	10 - 13 Other liquids and solids
WGK	WGK 1 slightly hazardous to water
Disposal	3 Relatively unreactive organic reagents should be collected in container A. If halogenated, they should be collected in container B. For solid residues use container C.

Storage and Shipping Information
Storage	Store at +2 °C to +8 °C.

Transport Information
Declaration (railroad and road) ADR, RID	Kein Gefahrgut
Declaration (transport by air) IATA-DGR	No Dangerous Good
Declaration (transport by sea) IMDG-Code	No Dangerous Good

Specifications
Appearance (clearness)	clear
Appearance (colour)	brown
pH-value (25 °C)	5.6 - 5.8
Solidification behaviour (2 hrs., 45 °C)	liquid
Growth promotion test in accordance with the current version of DIN EN ISO 11133.
Inoculum on reference medium (Lactobacillus acidophilus ATCC 4356 (WDCM 00098))
Inoculum on reference medium (Lactobacillus sakei ATCC 15521 (WDCM 00015))
Inoculum on reference medium (Lactococcus lactis spp. lactis ATCC 19435 (WDCM 00016))
Inoculum on reference medium (Pediococcus pentosaceus ATCC 33316 (WDCM 00158))
Inoculum on reference medium (Pediococcus damnosus ATCC 29358 (WDCM 00022))
Colony count (Lactobacillus acidophilus ATCC 4356 (WDCM 00098))
Colony count (Lactobacillus sakei ATCC 15521 (WDCM 00015))
Colony count (Lactococcus lactis spp. lactis ATCC 19435 (WDCM 00016))
Colony count (Pediococcus pentosaceus ATCC 33316 (WDCM 00158))
Colony count (Pediococcus damnosus ATCC 29358 (WDCM 00022))
Recovery on test medium (Lactobacillus sakei ATCC 15521 (WDCM 00015))	≥ 70 %
Recovery on test medium (Lactobacillus acidophilus ATCC 4356 (WDCM 00098))	≥ 70 %
Recovery on test medium (Lactococcus lactis spp. lactis ATCC 19435 (WDCM 00016))	≥ 70 %
Recovery on test medium (Pediococcus pentosaceus ATCC 33316 (WDCM 00158))	≥ 70 %
Recovery on test medium (Pediococcus damnosus ATCC 29358 (WDCM 00022))	≥ 70 %
Growth (Escherichia coli ATCC 25922 (WDCM 00013))	total inhibition
Growth (Escherichia coli ATCC 8739 (WDCM 00012))	total inhibition
Growth (Bacillus cereus ATCC 11778 (WDCM 00001))	total inhibition
Growth (Bifidobacterium bifidum ATCC 11863)	good growth
Incubation: 72 ± 3 hours at 30 ± 1 °C aerobic; Bifidobacterium anaerobic A recovery rate of 70 % is equivalent to a productivity value of 0.7. The indicated colony counts result from the sum of a triple determination. Reference media: MRS-Agar

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Merck 110660 MRS Agar (De Man, Rogosa And Sharpe) Acc. Iso 15214 granucult® 500 gr

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