Roche PHOSS-RO PhosSTOP™ sufficient for 10 10mL buffer preparations, sufficient for 20 10mL buffer preparations, suitable for tissue processing, suitable for immunoprecipitation (IP) 20 Tablets
Marka
Stok Kodu
LB.SA.4906837001
Kısa Bilgi
Synonym(s): inhibitor tablets for phosphatase, phosphatase inhibitor tablets
| Roche PHOSS-RO PhosSTOP™ |
|
Synonym(s): inhibitor tablets for phosphatase, phosphatase inhibitor tablets |
|
PROPERTIES
form tablet
usage
sufficient for 10 10 mL buffer preparations
sufficient for 20 10 mL buffer preparations
packaging
pkg of 10 tablets (04906845001)
pkg of 20 tablets (04906837001)
manufacturer/tradename Roche
technique(s)
immunoprecipitation (IP): suitable
tissue processing: suitable
solubility aqueous buffer: soluble
storage temp. 2-8°C
DESCRIPTION
General description
PhosSTOP tablets are a proprietary blend of phosphatase inhibitors, formulated as a ready-to-use tablet which dissolves quickly in aqueous solutions (buffers), including buffers containing formalin (for formalin fixation).
Specificity
Protects proteins against dephosphorylation. Inhibits phosphatase classes such as acid and alkaline phosphatases, as well as serine/threonine (PP1, PP2A, and PP2B) and tyrosine protein phosphatases (PTP) in bacterial, mammalian, yeast, and plant extracts.
Application
PhosSTOP™ has been used as a component of
lysis buffer for the lysis of frozen mouse tissue to obtain protein supernatant
lysis of bone derived macrophages
nuclear extraction buffer for cell lysis and immunoprecipitation
PhosSTOP™ protects protein extracts against dephosphorylation. The tablets inhibit a broad spectrum of phosphatases such as acidic and alkaline phosphatases, as well as serine/threonine (PP1, PP2A, and PP2B), dual-specificity phosphatases and tyrosine protein phosphatases (PTP) in bacterial, insect, mammalian, yeast, and plant extracts. PhosSTOP Tablets are well suited for the purification and detection of phoshorylated proteins. They are also used to prevent dephosphorylation in formalin-fixed paraffin embedded (FFPE) tissue sections. PhosSTOP tablets can be applied in combination with cOmplete Protease Inhibitor Cocktail Tablets to simultaneously protect proteins against dephosphorylation and proteolytic degradation. Both inhibitor tablets maintain their activity when used together. PhosSTOP inhibitors were evaluated for their effect on protein assays. The inhibition shows almost no influence on BCA or Bradford protein assays. |
