Sigma-Aldrich 116216 Peroxidase (from horse radish) 10000 U/vial for biochemistry EC 1.11.1.7 1 VIAL
Marka
Stok Kodu
LB.SA.1162160001
Kısa Bilgi
Synonym(s): Peroxidase CAS Number: 9003-99-0 EC Index Number: 232-668-6
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Sigma-Aldrich 116216 Peroxidase (from horse radish) 10000 U/vial for biochemistry |
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Synonym(s): Peroxidase
CAS Number: 9003-99-0
EC Index Number: 232-668-6
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PROPERTIES
biological source horseradish
Quality Level 200
form solid
pH 6.5-7.5 (20 °C, 10 g/L in H2O)
storage temp. 2-8°C
DESCRIPTION
General description
Horseradish peroxidase (HRP) is a glycoprotein that belongs to the class of oxidoreductase of the peroxidases family. It is found in horseradish roots. HRP contains significant amounts of carbohydrates and photohemin IX as its prosthetic group.
Packaging
1 vial in Glass bottle
Biochem/physiol Actions
Peroxidases catalyze the cleavage of peroxides by redox reactions. The active center includes heme, cysteine thiols, selenium, manganese, and other chemical moieties. Peroxidase isoenzymes are essential for oxidizing reactive oxygen species, innate immunity, hormone biosynthesis, and the pathogenesis of many diseases. They are generally used for sensors, drug delivery devices, environment protection, bioremediation, and energy production. Horseradish peroxidase (HRP) is the widely used oxidoreductase in colorimetric, fluorimetric, chemiluminescent, electrochemical, and immunoassays. Its concentration can be measured with O-phenylenediamine (OPD) and hydrogen peroxide. HRP serves as a tracer for immunocytochemistry and is widely used in antibody-enzyme conjugates.
Features and Benefits
Ideal for preparing small-sized antibody-enzyme conjugates
Enables better cellular penetration through low-molecular-weight conjugates
Small conjugate size ensures greater accessibility to antigenic structures within tissue sections
Stable even under the conditions employed for crosslinking
Analysis Note
Activity
- Hydrogen peroxide and guajacol; pH 7.0; 25 °C:
about 170 U/mg : ≥ 10000 U/Glas
Catalase: ≤ 1 U/mg |