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Sigma-Aldrich 67309 Kovac′s reagent for indoles suitable for microbiology 100 mL

Sigma-Aldrich 67309 Kovac′s reagent for indoles suitable for microbiology 100 mL
Synonym(s): 4-(Dimethylamino)benzaldehyde solution
Empirical Formula (Hill Notation): C9H11NO
CAS Number: 100-10-7
Molecular Weight: 149.19
Beilstein: 4132845
MDL number: MFCD00003381
PubChem Substance ID: 329760541
NACRES: NA.85
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Sigma-Aldrich 67309 Kovac′s reagent for indoles suitable for microbiology 100 mL  
PROPERTIES
Agency according to ISO 16654:2001 
Quality Level 200 
product line BioChemika 
shelf life limited shelf life, expiry date on the label 
composition
4-(dimethlyamino)benzaldehyde, 50 g/L
hydrochloric acid, 240 g/L
isoamylic alcohol, 710 g/L 
technique(s) microbe id | specific enzyme detection: suitable 
application(s)
agriculture
clinical testing
environmental food and beverages
microbiology 
storage temp. 2-8 °C 
suitability Escherichia coli coliforms 
SMILES string [H]C(=O)c1ccc(cc1)N(C)C 
InChI 1S/C9H11NO/c1-10(2)9-5-3-8(7-11)4-6-9/h3-7H,1-2H3 
InChI key BGNGWHSBYQYVRX-UHFFFAOYSA-N
 
DESCRIPTION 
General description
Kovac′s reagent is prepared by mixing p-dimethylaminobenzaldehyde, isoamyl alcohol and concentrated hydrochloric acid. For identification of an organism, the formation of Indole from a tryptophan substrate is a useful diagnostic tool. Indole production is a crucial test in identification of Escherichia coli. After incubation, adding the reagent will help in determination if Indole that has been produced. The reagent turns red when reacts with Indole.
Application
In the presence of oxygen, some bacteria, like E.coli, are able to split tryptophan into indole and alpha-aminopropionic acid. This reagent is for detecting the indole and identify the indole-positive and indole-negative microorganisms.
Kovac′s reagent may be used for spot test for determination of indole by saturating filter paper with the reagent. It may also be used for initial spot test for determination of indole followed by quantification by spectrophotometric assay and HPLC-UV/Vis-MS/MS methods.
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Sigma-Aldrich 67309 Kovac′s reagent for indoles suitable for microbiology 100 mL Sigma-Aldrich 67309 Kovac′s reagent for indoles suitable for microbiology 100 mL LB.SA.67309-100ML-F
Sigma-Aldrich 67309 Kovac′s reagent for indoles suitable for microbiology 100 mL

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