Sigma-Aldrich 67309 Kovac′s reagent for indoles suitable for microbiology 100 mL
Marka
Stok Kodu
LB.SA.67309-100ML-F
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Sigma-Aldrich 67309 Kovac′s reagent for indoles suitable for microbiology 100 mL |
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Synonym(s): 4-(Dimethylamino)benzaldehyde solution
Empirical Formula (Hill Notation): C9H11NO
CAS Number: 100-10-7
Molecular Weight: 149.19
Beilstein: 4132845
MDL number: MFCD00003381
PubChem Substance ID: 329760541
NACRES: NA.85
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PROPERTIES
Agency according to ISO 16654:2001
Quality Level 200
product line BioChemika
shelf life limited shelf life, expiry date on the label
composition
4-(dimethlyamino)benzaldehyde, 50 g/L
hydrochloric acid, 240 g/L
isoamylic alcohol, 710 g/L
technique(s) microbe id | specific enzyme detection: suitable
application(s)
agriculture
clinical testing
environmental food and beverages
microbiology
storage temp. 2-8 °C
suitability Escherichia coli coliforms
SMILES string [H]C(=O)c1ccc(cc1)N(C)C
InChI 1S/C9H11NO/c1-10(2)9-5-3-8(7-11)4-6-9/h3-7H,1-2H3
InChI key BGNGWHSBYQYVRX-UHFFFAOYSA-N
DESCRIPTION
General description
Kovac′s reagent is prepared by mixing p-dimethylaminobenzaldehyde, isoamyl alcohol and concentrated hydrochloric acid. For identification of an organism, the formation of Indole from a tryptophan substrate is a useful diagnostic tool. Indole production is a crucial test in identification of Escherichia coli. After incubation, adding the reagent will help in determination if Indole that has been produced. The reagent turns red when reacts with Indole.
Application
In the presence of oxygen, some bacteria, like E.coli, are able to split tryptophan into indole and alpha-aminopropionic acid. This reagent is for detecting the indole and identify the indole-positive and indole-negative microorganisms.
Kovac′s reagent may be used for spot test for determination of indole by saturating filter paper with the reagent. It may also be used for initial spot test for determination of indole followed by quantification by spectrophotometric assay and HPLC-UV/Vis-MS/MS methods. |
