Sigma-Aldrich 137270 HEPES EMPROVE® EVOLVE 25 kg
Kategori
Marka
Stok Kodu
LB.SA.1372709025
Kısa Bilgi
Synonym(s): HEPES, 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid) Empirical Formula (Hill Notation): C8H18N2O4S CAS Number: 7365-45-9 Molecular Weight: 238.30 Beilstein: 883043 MDL number: MFCD00006158
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Sigma-Aldrich 137270 HEPES EMPROVE® EVOLVE 25 kg
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| Synonym(s): HEPES, 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid) Empirical Formula (Hill Notation): C8H18N2O4S CAS Number: 7365-45-9 Molecular Weight: 238.30 Beilstein: 883043 MDL number: MFCD00006158 |
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PROPERTIES
biological source synthetic
Quality Level 400 product line EMPROVE® EVOLVE form powder technique(s) cell culture | mammalian: suitable impurities Endotoxin, microbial, and trace metals; tested pH 4.7-5.5 useful pH range 6.8-8.2 6.8-8.2 pKa (25 °C) 7.5 pKa 7.5 suitability suitable for manufacturing use foreign activity Cytotoxicity, DNase, NICKase, RNase, and Protease; tested storage temp. 2-30°C SMILES string OCCN1CCN(CC1)CCS(O)(=O)=O InChI 1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14) InChI key JKMHFZQWWAIEOD-UHFFFAOYSA-N DESCRIPTION
Application
HEPES is a biological buffer often referred to as a “Good′s” buffer. It is the most commonly used biological buffer since the pKa of HEPES is 7.31 which corresponds to the physiological pH of 7.36. HEPES is considered to be non-toxic to culture cell lines, highly water soluble and provides high solution clarity.HEPES is used in cell culture media, in biopharma buffer formulations (both upstream and downstream), and in diagnostic reagents. HEPES based buffers are used in purification bioprocesses of antibodies, bioactives, proteins and growth factors. Packaging 100 g in poly bottle 5, 10, 25, 50 kg in poly drum Legal Information Emprove is a registered trademark of Merck KGaA, Darmstadt, Germany |
