Sigma-Aldrich 198161 Toluidine Blue O certified by the Biological Stain Commission 25 gr
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Stok Kodu
LB.SA.198161-25G
| Sigma-Aldrich 198161 Toluidine Blue O certified by the Biological Stain Commission 25 gr |
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Synonym(s): Basic Blue 17, Blutene chloride, Methylene Blue T50 or T extra, Tolonium chloride
Empirical Formula (Hill Notation): C15H16ClN3S
CAS Number: 92-31-9
Molecular Weight: 305.83
Colour Index Number: 52040
EC Number: 202-146-2
MDL number: MFCD00011934
PubChem Substance ID: 24851865
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PROPERTIES
grade certified by the Biological Stain Commission
Quality Level 200
form powder
composition Dye content, 80%
λmax 626 nm
ε (extinction coefficient)
≥13000 at 241-247 nm in H2O
≥28000 at 287-293 nm in H2O
≥40000 at 625-635 nm in H2O
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp. room temp
SMILES string [Cl-].Cc1cc2N=C3C=CC(\C=C3Sc2cc1N)=[N+](\C)C InChI 1S/C15H15N3S.ClH/c1-9-6-13-15(8-11(9)16)19-14-7-10(18(2)3)4-5-12(14)17-13;/h4-8,16H,1-3H3;1H
InChI key GEDVVYWLPUPJJZ-UHFFFAOYSA-N
DESCRIPTION
General description
A metachromatic dye suitable for a wide variety of histological staining procedures.
Application
Toluidine Blue O along with light-emitting diode has been used as a photosensitizer to control in vitro biofilms and to kill gram-negative bacteria.
Packaging
5, 25 g in glass bottle
Biochem/physiol Actions
Toluidine Blue O (TBO) is a thiazine dye of the quinone-imine family and is cationic in nature. It has been employed in polychromatic staining of paraffin embedded plant cell walls. TBO can be removed from its aqueous solution by using an effective adsorbent, clinoptilolite. TBO and fixed output laboratory laser light source are used together to destroy variety of oral bacteria associated with dental caries. This metachromatic nuclear stain is often used in place of thionin or methylene blue. It also serves as a component of a tribasic stain along with Malachite Greenand Basic Fuchsin.
Suitability
Certified for use as a metachromatic stain for mast cells and cartilage using the Churukian-Schenk method and the method of Nocht on paraffin sections. |
