Sigma-Aldrich 318337 Acridine Orange hydrochloride hydrate ≥98% (HPLC) 1 gr
Kategori
Marka
Stok Kodu
LB.SA.318337-1G
Kısa Bilgi
Synonym(s): 3,6-Bis(dimethylamino)acridine hydrochloride Empirical Formula (Hill Notation): C17H19N3 · HCl · xH2O CAS Number: 65-61-2 Molecular Weight: 301.81 (anhydrous basis) Colour Index Number: 46005 Beilstein: 3734978 EC Number: 200-614-0 MDL number: MFCD00150070 PubChem Substance ID: 24859123 NACRES: NA.47
Garanti Süresi
24 Ay
| Sigma-Aldrich 318337 Acridine Orange hydrochloride hydrate ≥98% (HPLC) 1 gr |
|
Synonym(s): 3,6-Bis(dimethylamino)acridine hydrochloride
Empirical Formula (Hill Notation): C17H19N3 · HCl · xH2O
CAS Number: 65-61-2
Molecular Weight: 301.81 (anhydrous basis)
Colour Index Number: 46005
Beilstein: 3734978
EC Number: 200-614-0
MDL number: MFCD00150070
PubChem Substance ID: 24859123
NACRES: NA.47 |
|
PROPERTIES
Quality Level 200
Assay ≥98% (HPLC)
form powder
mp 284-287 °C (lit.)
solubility H2O: 0.1%, clear, orange to red
λmax 492 nm
ε (extinction coefficient)
≥14,000 at 227-233 nm in water at 0.005 g/L
≥35000 at 265-271 nm in water at 0.005 g/L
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp. room temp
SMILES string Cl[H].[H]O[H].CN(C)c1ccc2cc3ccc(cc3nc2c1)N(C)C
InChI 1S/C17H19N3.ClH.H2O/c1-19(2)14-7-5-12-9-13-6-8-15(20(3)4)11-17(13)18-16(12)10-14;;/h5-11H,1-4H3;1H;1H2
InChI key JUAAUEXLEKAEQC-UHFFFAOYSA-N
DESCRIPTION
Application
A rigorously purified zinc-free material suitable for use in flow cytometry. Acridine Orange hydrochloride hydrate dye has been used:
- to stain intracellular acidic vesicles.[1]
- to measure lysosomal membrane permeability (LMP).[1]
- or autophagy detection.[2]
Fluorescent stain for nucleic acids in agarose and polyacrylamide gels. Has been used to study apoptosis in various cell culture and tissue models.
Biochem/physiol Actions
Acridine Orange is a metachromatic dye which can stain DNA, RNA and acid glycosaminoglycans. At low concentrations, it intercalates into DNA and precipitates RNA. However, at high concentrations it denatures and precipitates both RNA and DNA.[3] Acridine Orange is also used to analyze autophagy. It enters into acidic organelles in a pH-dependent manner. At neutral pH, acridine orange gives a green fluorescence and in acidic conditions, it accumulates in acidic organelles giving a bright red fluorescence.[4]
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