Sigma Aldrich 48305 Bile salts suitable for microbiology 250 gr
Marka
Stok Kodu
LB.SA.48305-250G-F
Kısa Bilgi
Synonym(s): Bile acids sodium salt, cholic acid-deoxycholic acid sodium salt mixture, Bile acids sodium salt, Cholic acid-Deoxycholic acid sodium salt mixture MDL number: MFCD00130648 NACRES: NA.85
| Sigma Aldrich 48305 Bile salts suitable for microbiology 250 gr |
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Synonym(s): Bile acids sodium salt, cholic acid-deoxycholic acid sodium salt mixture, Bile acids sodium salt, Cholic acid-Deoxycholic acid sodium salt mixture
MDL number: MFCD00130648
NACRES: NA.85 |
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PROPERTIES
description anionic
Quality Level 200
sterility non-sterile
Assay 40-60% (Cholic Acid ; approximately 50% sodium cholate and approximately 50% sodium deoxycholate, HPLC)
form powder
composition
cholic acid sodium salt, ~50%
deoxycholic acid sodium salt, ~50%
impurities ≤5% water
loss ≤5% loss on drying (water)
solubility H2O: 0.1 g/mL, clear, faintly yellow
application(s) microbiology
storage temp. 10-30°C
DESCRIPTION
General description
Bile salt is an organic sodium salt with a conjugate of any of the bile acids along with either glycine or taurine. Biles salts are composed of four different kinds of bile acids - cholic, deoxycholic, chenodeoxycholic and lithocholic acids. Bile salts are extracted under controlled conditions from purified fresh bile, to be used in bacteriological culture media, where it behaves as a selective inhibitory agent that enables isolation and identification of pathogens. For example, in MacConkey Agar/Broth.
Application
Bile salts may be used as one of the selective agents to prevent the growth of injured Escherichia coli cells, in an experiment done in order to identify the specific agent in the selective media which would inhibit the growth of the injured Escherichia coli ML30 cells.[1] It may also be used for identification of isolates of Vibrio (Beneckea) vulnificus which is a halophilic bacterium, found responsible for causing several human infections.[2]
Biochem/physiol Actions
Bile salts are surface-active steroid compounds and their primary physiological function is to aid in the digestion of lipophilic nutrients in intestinal tracts of vertebrates.[3] |
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