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Sigma-Aldrich 75605 m-CP Agar Base suitable for microbiology, NutriSelect® Plus 500 gr |
Synonym(s): Membrane Clostridium perfringens Agar Base UNSPSC Code: 41171606 NACRES: NA.85 |
PROPERTIES
sterility non-sterile
Quality Level 200 form powder shelf life limited shelf life, expiry date on the label composition agar, 15 g/L bromocresol purple, 0.04 g/L L-cysteine hydrochloride, 1 g/L ferric chloride 6H2O, 0.09 g/L indoxyl-β-D-glucoside, 0.06 g/L magnesium sulfate 7H2O, 0.1 g/L sucrose, 5 g/L tryptose, 30 g/L yeast extract, 20 g/L manufacturer/tradename NutriSelect® Plus technique(s) microbiological culture: suitable final pH 7.6±0.2 (25 °C) application(s) environmental food and beverages water monitoring microbiology storage temp. 2-8°C suitability selective and differential for Clostridium spp. DESCRIPTION Application The m-CP Agar Base with selective supplement is recommended by the Directive of the Council of the European Union 98/83/EC for isolation and enumeratin of Clostridium perfringens from water samples using membrane filtration technique. Preparation Note Suspend 35.6 g in 485 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 1 bar pressure (121 °C) for 15 minutes. Cool to 50 °C. Aseptically add the rehydrated contents of 1 vial of M-CP Selective Supplement I (Cat. No. 51962) and 1 vial of M-CP Selective Supplement II (Cat. No. 82265). Mix well and pour into sterile petri plates. Other Notes Medium for the assay of water for Clostridium perfringens[1]; EU 98/83/EC Council 3rd November 1998 on quality of water intended for human consumption[2] Footnote We offer two media types: the superior granulated GranuCult and the cost-efficient powdered NutriSelect culture media, depending on your needs. The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance. Legal Information GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany |