Sigma-Aldrich 62971 Lysozyme from chicken egg white powder (crystalline), ~70000 U/mg
Marka
Stok Kodu
LB.SA.62971
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Sigma-Aldrich 62971 Lysozyme from chicken egg white |
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Synonym(s): Mucopeptide N-acetylmuramoylhydrolase, Muramidase
CAS Number: 12650-88-3
Enzyme Commission number: 3.2.1.17 (BRENDA, IUBMB)
EC Number: 235-747-3
MDL number: MFCD00131557
NACRES: NA.22
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PROPERTIES
form powder (crystalline)
Quality Level 100
specific activity ~70000 U/mg
mol wt single-chain 14.3 kDa, Mr ~14600
UniProt accession no. P00698
storage temp. 2-8 °C
Gene Information chicken... LYZ(396218)
DESCRIPTION
Application
Enzyme breaks down the cell walls of bacteria; used to prepare spheroplasts.
Biochem/physiol Actions
Lysozyme hydrolyzes β(1→4) linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrin. Gram-positive cells are quite susceptible to this hydrolysis as their cell walls have a high proportion of peptidoglycan. Gram-negative bacteria are less susceptible due to the presence of an outer membrane and a lower proportion of peptidoglycan. However, these cells may be hydrolyzed in the presence of EDTA that chelates metal ions in the outer bacterial membrane.
The enzyme is active over a broad pH range (6.0 to 9.0). At pH 6.2, maximal activity is observed over a wider range of ionic strengths (0.02 to 0.100 M) than at pH 9.2 (0.01 to 0.06 M).
Unit Definition
1 U corresponds to the amount of enzyme which decreases the absorbance at 450 nm by 0.001 per minute at pH 7.0 and 25°C (Micrococcus luteus, ATCC 4698, as substrate) |
