Sigma-Aldrich 93363 Trizma® hydrochloride BioUltra, for molecular biology, ≥99.0% (AT) 500 gr
Marka
Stok Kodu
LB.SA.93363-500G
Kısa Bilgi
Synonym(s): TRIS HCl, TRIS hydrochloride, Tris(hydroxymethyl)aminomethane hydrochloride, Tromethane hydrochloride Linear Formula: NH2C(CH2OH)3 · HCl CAS Number: 1185-53-1 Molecular Weight: 157.60 Beilstein: 3675235 EC Number: 214-684-5 MDL number: MFCD00012590 PubChem Substance ID: 57653541 NACRES: NA.25
Bilgi
CAS Number: 1185-53-1
| Sigma-Aldrich 93363 Trizma® hydrochloride BioUltra, for molecular biology, ≥99.0% (AT) |
| Synonym(s): TRIS HCl, TRIS hydrochloride, Tris(hydroxymethyl)aminomethane hydrochloride, Tromethane hydrochloride Linear Formula: NH2C(CH2OH)3 · HCl CAS Number: 1185-53-1 Molecular Weight: 157.60 Beilstein: 3675235 EC Number: 214-684-5 MDL number: MFCD00012590 PubChem Substance ID: 57653541 NACRES: NA.25 |
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PROPERTIES
grade for molecular biology
product line BioUltra Assay ≥99.0% (AT) form powder or crystals impurities DNases, none detected RNases, none detected insoluble matter, passes filter test phosphatases, none detected proteases, none detected ign. residue ≤0.2% (as SO4) loss ≤0.2% loss on drying, 110 °C pH 2.5-4.0 (25 °C, 4 M in H2O) useful pH range 7.0-9.0 pKa (25 °C) 8.1 mp 150-152 °C solubility H2O: 4 M at 20 °C, clear, colorless anion traces sulfate (SO42-): ≤50 mg/kg cation traces Al: ≤5 mg/kg As: ≤0.1 mg/kg Ba: ≤5 mg/kg Bi: ≤5 mg/kg Ca: ≤10 mg/kg Cd: ≤5 mg/kg Co: ≤5 mg/kg Cr: ≤5 mg/kg Cu: ≤5 mg/kg Fe: ≤5 mg/kg K: ≤50 mg/kg Li: ≤5 mg/kg Mg: ≤5 mg/kg Mn: ≤5 mg/kg Mo: ≤5 mg/kg Na: ≤50 mg/kg Ni: ≤5 mg/kg Pb: ≤5 mg/kg Sr: ≤5 mg/kg Zn: ≤5 mg/kg λ 4 M in H2O UV absorption λ: 260 nm Amax: 0.12 λ: 280 nm Amax: 0.08 SMILES string Cl.NC(CO)(CO)CO InChI 1S/C4H11NO3.ClH/c5-4(1-6,2-7)3-8;/h6-8H,1-3,5H2;1H InChI key QKNYBSVHEMOAJP-UHFFFAOYSA-N DESCRIPTION Application Trizma is used in the formulation of buffer solutions in the pH range between 7.5 and 8.5. Tris buffer solutions are widely used in cell and molecular biology for processes such as protein and nucleic acid extraction and purification. Trizma based buffers are also in column chromatography and in gel electrophoresis. Trizma hydrochloride is used as a general reagent for the preparation of all types of Tris buffers. Other Notes Easily compare specifications for Trizma HCl products with the Trizma HCl specification table. The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution. For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode. Component of extraction buffer e.g. phenol extraction of DNA or RNA[1]; Buffer component of separating and stacking gels in the characterization of in vitro translation products by SDS-PAGE[2]. Legal Information Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany |
