Sigma-Aldrich 94403 Hoechst 33258 solution 1 mg/mL in H2O, ≥98.0% (HPLC) 1 mg
Marka
Stok Kodu
LB.SA.94403-1ML
Kısa Bilgi
Synonym(s): bisBenzimide H 33258 solution Empirical Formula (Hill Notation): C25H24N6O · 3HCl CAS Number: 23491-45-4 Molecular Weight: 533.88 PubChem Substance ID: 329770334 NACRES: NA.32
Bilgi
CAS Number: 23491-45-4
| Sigma-Aldrich 94403 Hoechst 33258 solution 1 mg/mL in H2O, ≥98.0% (HPLC) 1 mg |
| Synonym(s): bisBenzimide H 33258 solution Empirical Formula (Hill Notation): C25H24N6O · 3HCl CAS Number: 23491-45-4 Molecular Weight: 533.88 PubChem Substance ID: 329770334 NACRES: NA.32 |
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PROPERTIES
Quality Level 100
Assay ≥98.0% (HPLC) form liquid concentration 1 mg/mL in H2O solubility DMF: soluble, H2O: soluble fluorescence λex 355 nm; λem 465 nm in TE buffer; DNA storage temp. 2-8°C SMILES string Cl.Cl.Cl.CN1CCN(CC1)c2ccc3[nH]c(nc3c2)-c4ccc5nc([nH]c5c4)-c6ccc(O)cc6 InChI 1S/C25H24N6O.3ClH/c1-30-10-12-31(13-11-30)18-5-9-21-23(15-18)29-25(27-21)17-4-8-20-22(14-17)28-24(26-20)16-2-6-19(32)7-3-16;;;/h2-9,14-15,32H,10-13H2,1H3,(H,26,28)(H,27,29);3*1H InChI key SMNPLAKEGAEPJD-UHFFFAOYSA-N DESCRIPTION General description Hoechst 33258, also known as bisbenzimide, is a fluorescent blue dye. [1]It is categorized as a chromosomal stain that binds to the minor groove of the DNA double helix recognizing a run of four A-T base pairs.[2] Application Hoechst 33258 is used to estimate DNA concentration in samples by fluorometry which is better than spectrophotometry, allowing the determination of nanogram quantities of DNA.[3] Also, Hoechst 33258 is suitable to detect mycoplasma contamination in cell cultures due to its high fluorescence ability.[4] |