Sigma-Aldrich A3678 Ammonium persulfate for molecular biology, suitable for electrophoresis, ≥98% 25 gr
Marka
Stok Kodu
LB.SA.A3678-25G
Kısa Bilgi
Sigma-Aldrich A3678 Amonyum persülfat; Synonym(s): AP, APS, Ammonium peroxodisulfate, Ammonium peroxydisulfate, PER Linear Formula: (NH4)2S2O8 CAS Number: 7727-54-0 Molecular Weight: 228.20 EC Number: 231-786-5 MDL number: MFCD00003390
| Sigma-Aldrich A3678 Ammonium persulfate for molecular biology, suitable for electrophoresis, ≥98% 25 gr |
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Synonym(s): AP, APS, Ammonium peroxodisulfate, Ammonium peroxydisulfate, PER
Linear Formula: (NH4)2S2O8
CAS Number: 7727-54-0
Molecular Weight: 228.20
EC Number: 231-786-5
MDL number: MFCD00003390
eCl@ss: 38030421
PubChem Substance ID: 24890800
NACRES: NB.22
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PROPERTIES
grade for molecular biology
Quality Level 200
vapor density 7.9 (vs air)
assay ≥98%
form powder
reaction suitability reagent type: oxidant
technique(s) electrophoresis: suitable
anion traces
chloride (Cl-): <10 ppm
cation traces
Fe: <10 ppm
heavy metals (as Pb): <50 ppm
foreign activity
DNAse, none detected
Protease, none detected
RNAse, none detected
SMILES string N.N.OS(=O)(=O)OOS(O)(=O)=O
InChI 1S/2H3N.H2O8S2/c;;1-9(2,3)7-8-10(4,5)6/h2*1H3;(H,1,2,3)(H,4,5,6)
InChI key ROOXNKNUYICQNP-UHFFFAOYSA-N
DESCRIPTION
General description
Ammonium persulfate (APS) is a widely used reagent in biochemistry and molecular biology for the preparation of polyacrylamide gels. APS forms oxygen free radicals in aqueous solution by a base-catalyzed mechanism. The bases, most commonly used as catalysts, are tertiary amines such as TEMED (N,N,N′,N′-tetramethylethylenediamine) or DMAPN (3-dimethylaminopropionitrile). The free radicals will cause the polymerization of acrylamide and bis-acrylamide to form a gel matrix, which can be used for separating macromolecules by size.[1] APS has also been utilized to study protein-protein interactions via photoinitiated crosslinking chemistry.[2]
Application
Ammonium persulfate has been used for the preparation of polyacrylamide gels and acrylamide hydrogels.[3][4][5] Catalyst for acrylamide gel polymerization.
Packaging
25, 100 g in poly bottle
Analysis Note
Tested for use in acrylamide polymerization. |
