Sigma-Aldrich A9539 Agarose BioReagent, for molecular biology, low EEO 250 gr
Marka
Stok Kodu
LB.SA.A9539-250G
Bilgi
CAS Number: 9012-36-6
| Sigma-Aldrich A9539 Agarose BioReagent, for molecular biology, low EEO 250 gr |
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CAS Number: 9012-36-6
EC Number: 232-731-8
MDL number: MFCD00081294
PubChem Substance ID: 24891469
NACRES: NA.25
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PROPERTIES
biological source algae (marine)
Quality Level 200
grade for molecular biology
product line BioReagent
form powder
technique(s) electrophoresis: suitable
impurities ≤10% moisture content
EEO 0.09-0.13
transition temp gel point 36 °C ±1.5 °C (1.5% gel)
gel strength ≥1200 g/cm2 (1% gel)
anion traces sulfate (SO42-): ≤0.15%
foreign activity DNase, RNase, none detected
InChI 1S/C24H38O19/c25-1-5-9(27)11(29)12(30)22(38-5)41-17-8-4-36-20(17)15(33)24(40-8)43-18-10(28)6(2-26)39-23(14(18)32)42-16-7-3-35-19(16)13(31)21(34)37-7/h5-34H,1-4H2/t5-,6-,7+,8+,9+,10+,11+,12-,13+,14-,15+,16-,17-,18+,19+,20+,21-,22+,23+,24+/m1/s1
InChI key MJQHZNBUODTQTK-WKGBVCLCSA-N
DESCRIPTION
Packaging
10, 25, 50, 100, 250, 500 gr in poly bottle
Application
Routine use agarose is ideal for everyday analysis of nucleic acids by gel electrophoresis or blotting (Northern or Southern) and is also suitable for protein applications such as Ouchterlony and radial immunodiffusion (RID). Has low ethidium bromide and SYBR Green background staining.
Analysis Note
The following is a list of properties associated with our agaroses: Sulfate content - used as an indicator of purity, since sulfate is the major ionic group present. Gel strength - the force that must be applied to a gel to cause it to fracture. Gel point - the temperature at which an aqueous agarose solution forms a gel as it cools. Agarose solutions exhibit hysteresis in the liquid-to-gel transition - that is, their gel point is not the same as their melting temperature. Electroendosmosis (EEO) - a movement of liquid through the gel. Anionic groups in an agarose gel are affixed to the matrix and cannot move, but dissociable counter cations can migrate toward the cathode in the matrix, giving rise to EEO. Since electrophoretic movement of biopolymers is usually toward the anode, EEO can disrupt separations because of internal convection.
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