Sigma-Aldrich APF Alkaline Phosphatase Detection Kit, Fluorescence 1 KIT
| Sigma-Aldrich APF Alkaline Phosphatase Detection Kit, Fluorescence 1 KIT |
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EC Number: 245-325-0
eCl@ss: 42010105
NACRES: NA.53
SDS İçin Resmi Tıklayınız... |
| PROPERTIES
grade for molecular biology
Quality Level 200
usage kit sufficient for 300 reactions (200 μl reactions)
shipped in wet ice
storage temp. −20 °C
Gene Information human... ALPL(249)
DESCRIPTION
General description
The Alkaline Phosphatase (AP) Kit uses a fluorometric assay to detect AP activity. The kit capability is linear over a wide range of enzyme concentrations, which makes it particularly well suited for comparative analysis. The gene for alkaline phosphatase (AP) commonly serves as a reporter gene and is used to determine the strength of promoters and enhancers, define the role of transcription factors, assess transfection efficiency and measure the success of molecular cloning attempts. The alkaline phosphatase (AP) kit uses a fluorometric assay to detect AP activity. The kit capability is linear over a wide range of enzyme concentrations, which makes it particularly well suited for comparative analysis.
Application
Alkaline Phosphatase Detection Kit, Fluorescence has been used to determine alkaline phosphatase (ALP) activity. Suitable for measuring the alkaline phosphatase (AP) or secreted alkaline phosphatase (SEAP)reporter gene activity. The Alkaline Phosphatase Kit can be used to determine the strength of promoters and enhancers, define the role of transcription factors, assess transfection efficiency, and measure the success of molecular cloning attempts.
Also suitable for detection of AP activity in pluripotent stem cells, including embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs).
Biochem/physiol Actions
Alkaline phosphatase (ALP) helps in hydrolyses and transphosphorylation of several phosphoric acid monoesters. It modulates various intracellular processes, that are associated with cell cycle, apoptosis, growth and signal transduction pathways.
Features and Benefits
Up to 100x more sensitive than colorimetric assays
SEAP can be measured without sample destrutcion
Rapid and reproducible results
Safe and easy-to-use
Components
The Alkaline Phosphatase Kit contains:
50 mL Fluorescent Assay Buffer ( B6558)
6 mL Dilution Buffer (B6433)
50 μL Alkaline Phosphatase Control Enzyme, 0.1 mg/mL (C9361)
2 x 1 mg 4-Methylumbelliferyl phosphate disodium (M8168) |