Sigma-Aldrich B3801 Vitamin B12 assay medium NutriSelect® Plus, suitable for microbiology 100 gr
Marka
Stok Kodu
LB.SA.B3801-100G
Kısa Bilgi
Synonym(s): Vitamin B12 Assay Medium, Cyanocobalamin Assay Medium NACRES: NA.85
| Sigma-Aldrich B3801 Vitamin B12 assay medium NutriSelect® Plus 100 gr |
|
Synonym(s): Vitamin B12 Assay Medium, Cyanocobalamin Assay Medium
NACRES: NA.85 |
|
PROPERTIES
sterility non-sterile
Quality Level 200
form powder
shelf life limited shelf life, expiry date on the label
packaging bottle of 100 g
manufacturer/tradename NutriSelect® Plus
technique(s) microbiological culture: suitable
color light amber
final pH 6.1±0.2 (25 °C)
application(s)
agriculture, food and beverages, pharmaceutical, microbiology
storage temp. 2-8°C
suitability nonselective for Lactobacillus spp.
DESCRIPTION
General description
Vitamin B12 Assay Medium is a Vitamin B12 free medium containing all other vitamins and nutrients essential for the growth of Lactobacillus leichmannii ATCC 7830 as the test organism. Cyanocobalamin (Vitamin B12, Cat.No. 95190 ) is added in increasing concentrations giving a growth response that can be measured turbidimetrically or acidimetrically. A standard curve is plotted with absorbance as a function of the vitamin B12 concentration. The concentration of vitamin B12 in the test sample is calculated based on the interpretation of the standard curve. Lactobacillus species grow poorly on non-selective culture media and require special nutrients for their growth. Vitamin assay media are prepared for use in the microbiological assay of vitamins. Three types of media used for the microbiological assay of vitamins are: - The maintenance media used for carrying the stock culture
- The inoculum media for preparation of the inoculum
- The assay media for quantitation of the vitamin under test.
Application
Vitamin B12 Assay Medium is a Vitamin B12 free medium recommended for microbiological assay of Vitamin B12 from various test samples
Features and Benefits
NutriSelect® Plus are powdered culture media offering
- Quality control with growth promotion test according to industry standards
- Quality assurance acc. to ISO 9001
- Cost-efficient powdered media
Components
Ingredients (g/L)
Casein acid hydrolysate, 15.00
Dextrose, 40.00
Asparagine, 0.20
Sodium acetate, 20.00
Ascorbic acid, 4.00
L-Cystine, 0.40
DL-Tryptophan, 0.40
Adenine sulfate, 0.02
Guanine hydrochloride, 0.02
Uracil, 0.02 Xanthine, 0.02
Riboflavin (Vitamin B2), 0.001
Thiamine hydrochloride, 0.001
Biotin, 0.00001
Niacin, 0.002
p-Amino benzoic acid, 0.002
Calcium pantothenate, 0.001
Pyridoxine hydrochloride, 0.004
Pyridoxal hydrochloride, 0.004
Pyridoxamine hydrochloride, 0.0008
Folic acid, 0.0002
Monopotassium phosphate, 1.00
Dipotassium phosphate, 1.00
Magnesium sulfate, 0.40
Sodium chloride, 0.02
Ferrous sulfate, 0.02
Manganese sulfate, 0.02
Polysorbate 80, 2.00
Preparation Note
Suspend 8.5 g of Vitamin B12 Assay Medium in 100 ml of distilled water. Boil to dissolve the medium completely. Mix well to distribute the slight precipitate evenly. Dispense 5 ml of medium into tubes containing increasing concentrations of cyanocobalamin (Cat. No. 43107) standard or the unknown. The total volume of 10 ml per tube is adjusted by adding distilled water. Sterilize by autoclaving at 15 lbs. pressure (121°C) for 15 minutes. Cool the medium.
Footnote
We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs. The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
Legal Information
GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany |
