Sigma-Aldrich B4882 Bromelain from pineapple stem ≥3 units/mg protein 100 gr
Marka
Stok Kodu
LB.SA.B4882-100G
| Sigma-Aldrich B4882 Bromelain from pineapple stem ≥3 units/mg protein 100 gr |
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CAS Number: 37189-34-7
Enzyme Commission number: 3.4.22.32 (BRENDA, IUBMB)
EC Number: 253-387-5
MDL number: MFCD00081423
NACRES: NA.54
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PROPERTIES
form lyophilized powder
Quality Level 200
specific activity ≥3 units/mg protein
composition Protein, ≥30% biuret
storage temp. −20°C
DESCRIPTION
Application
Bromelain from pineapple stem has been used:
to evaluate the aqueous micellar two-phase systems composed of ionic liquids as co-surfactants[1]
as a positive control in sodium dodecyl sulfate (SDS) / polyacrylamide gel electrophoresis (PAGE) and in cells proliferation assay[2]
to study its potential analgesic effect and to evaluate its hydrolyzingeffects on proenkephalin (PENK)-derived peptides containing enkephalin sequences[3]
Bromelain may be used to inhibit the biosysnthesis of proinflammatory prostaglandins. It may be used to reduce clotting efficiency. Bromelain, from pineapple stem, has been used to make enzymatic hydrolysates of honeybee-collected pollen[4].
Biochem/physiol Actions
Bromelain is a cysteine endopeptidase with broad specificity for cleavage of proteins. Bromelain may be from a stem or piece of fruit. Product B4882 is from pineapple stem. Product P3169 (pGlu-Phe-Leu-p-nitroanilide) may be used as a chromogenic substrate for the assay of bromelain.
Unit Definition
One unit will release 1.0 micromole of p-nitrophenol from N-alpha-CBZ-L-Lysine p-nitrophenyl ester per minute at pH 4.6 at 25 °C. One old titrimetric unit (pH 4.5 at 45 °C) is equivalent to approximately 1.7 new units (pH 4.6 at 25 °C).
Preparation Note
The product may be suspended in acetate buffer, pH 4.5 at 1 mg/mL concentration, yielding a hazy, off-white suspension.
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