Sigma-Aldrich C5070 CHAPS hydrate BioXtra, ≥98% (TLC) 1 gr
Marka
Stok Kodu
LB.SA.C5070-1G
Kısa Bilgi
Synonym(s): 3-[(3-Cholamidopropyl)dimethylammonio]-1-propanesulfonate hydrate, CHAPS Empirical Formula (Hill Notation): C32H58N2O7S · xH2O CAS Number: 75621-03-3 Molecular Weight: 614.88 (anhydrous basis) PubChem Substance ID: 24892750 NACRES: NA.21
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Sigma-Aldrich C5070 CHAPS hydrate BioXtra, ≥98% (TLC) 1 gr |
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Synonym(s): 3-[(3-Cholamidopropyl)dimethylammonio]-1-propanesulfonate hydrate, CHAPS
Empirical Formula (Hill Notation): C32H58N2O7S · xH2O
CAS Number: 75621-03-3
Molecular Weight: 614.88 (anhydrous basis)
PubChem Substance ID: 24892750
NACRES: NA.21
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PROPERTIES
description zwitterionic
Quality Level 400
product line BioXtra
assay ≥98% (TLC)
form powder
mol wt 614.88 g/mol (anhydrous basis)
technique(s)
protein purification: suitable
protein quantification: suitable
impurities
≤0.001% Phosphorus (P)
≤0.1% Insoluble matter
<0.04% DMF
ign. residue ≤0.1%
mp 157 °C (dec.) (lit.)
solubility H2O: 0.1 M, clear, colorless
anion traces chloride
(Cl-): ≤0.05%
cation traces
Al: ≤0.0005%
Ca: ≤0.0005%
Cu: ≤0.0005%
Fe: ≤0.0005%
K: ≤0.005%
Mg: ≤0.0005%
NH4+: ≤0.05%
Na: ≤0.005%
Pb: ≤0.001%
Zn: ≤0.0005%
SMILES string O.C[C@H](CCC(=O)NCCC[N+](C)(C)CCCS([O-])(=O)=O)[C@H]1CC[C@H]2[C@@H]3[C@H](O)C[C@@H]4C[C@H](O)CC[C@]4(C)[C@H]3C[C@H](O)[C@]12C
InChI 1S/C32H58N2O7S.H2O/c1-21(8-11-29(38)33-14-6-15-34(4,5)16-7-17-42(39,40)41)24-9-10-25-30-26(20-28(37)32(24,25)3)31(2)13-12-23(35)18-22(31)19-27(30)36;/h21-28,30,35-37H,6-20H2,1-5H3,(H-,33,38,39,40,41);1H2/t21-,22+,23-,24-,25+,26+,27-,28+,30+,31+,32-;/m1./s1
InChI key SJCUTFKCLFLIFE-JWTJKVBLSA-N
DESCRIPTION
General description
CHAPS is a non-denaturing, zwitterionic sulfobetaine detergent. It is mild and solubilizes membrane proteins and receptors. It is useful for breaking protein-protein interactions. It is a derivative of the naturally occurring bile salts.[1] It has small micellar molecular weight (6,150) and high critical micellar concentration (6 - 10 mM) allowing it to be removed from samples by dialysis.
Application
10mM CHAPS detergent has been used in the process of ion-exchange chromatography for improved purification.[2]
0.5 w/v of CHAPS is a component of high salt buffer in protein purification alongside HEPES, NaCl and EDTA.[3]
HEPES buffer has been used as lysis buffer in immunoprecipitation, supplemented with 1% CHAPS.[4]
Packaging
1, 5 g in poly bottle |