Sigma-Aldrich C7901 Chelex® 100 sodium form 50-100 mesh (dry) 25 gr
Marka
Stok Kodu
LB.SA.C7901-25G
Kısa Bilgi
Synonym(s): Iminodiacetic Acid Resin CAS Number: 11139-85-8 MDL number: MFCD00163980 NACRES: NA.56
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Sigma-Aldrich C7901 Chelex® 100 sodium form 50-100 mesh (dry) 25 gr |
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Synonym(s): Iminodiacetic Acid Resin
CAS Number: 11139-85-8
MDL number: MFCD00163980
NACRES: NA.56 |
|
PROPERTIES
form beads
matrix 1% cross-linked polystyrene, cross-linked polystyrene beads
matrix active group iminodiacetic acid (sodium form), paired iminodiacetate groups
matrix attachment through methyl group to aromatic rings
particle size 50-100 mesh (dry)
pH 4-14
capacity ≥0.3 meq/mL binding capacity, 0.6 meq/g binding capacity (heavy metal ions)
DESCRIPTION
Application
Chelex is used in affinity chromatography, protein chromatography, chelating resins, affinity resins and core bioreagents. Chelex® 100 sodium form has been used:
- for the extraction of DNA from bee samples[1]
- to remove from cigarette smoke extract (CSE) and study the effects of hepcidin expression in the murine lungs[2]
- to extract DNA from bacterial cultures[3]
Biochem/physiol Actions
Chelex® 100 is a styrene-divinylbenzene copolymer containing paired iminodiacetate ions, which act as chelating groups in binding polyvalent metal ions.[4] It is considered a weakly acidic resin. Chelex acts as a chelating resin that is used in DNA extraction from forensic samples, such as hair, bloodstain cards, and buccal swabs.[4]
Other Notes
Product is autoclavable in sodium form. It has a maximum operating temperature of 75°C and is not soluble in water.
Legal Information
Chelex is a registered trademark of Bio-Rad Laboratories, Inc. |
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