Sigma Aldrich D8130 5,5′-Dithiobis(2-nitrobenzoic acid) ≥98%, BioReagent, suitable for determination of sulfhydryl groups 500 mg
Marka
Stok Kodu
LB.SA.D8130-500MG
Kısa Bilgi
Synonym(s): 3-Carboxy-4-nitrophenyl disulfide, 6,6′-Dinitro-3,3′-dithiodibenzoic acid, Bis(3-carboxy-4-nitrophenyl) disulfide, DTNB, Ellman’s Reagent Linear Formula: [-SC6H3(NO2)CO2H]2 CAS Number: 69-78-3 Molecular Weight: 396.35 Beilstein: 1896821 EC Number: 200-714-4 MDL number: MFCD00007140 PubChem Substance ID: 24894189 NACRES: NA.26
| Sigma Aldrich D8130 5,5′-Dithiobis(2-nitrobenzoic acid) ≥98%, BioReagent |
|
Synonym(s): 3-Carboxy-4-nitrophenyl disulfide, 6,6′-Dinitro-3,3′-dithiodibenzoic acid, Bis(3-carboxy-4-nitrophenyl) disulfide, DTNB, Ellman’s Reagent
Linear Formula: [-SC6H3(NO2)CO2H]2
CAS Number: 69-78-3
Molecular Weight: 396.35
Beilstein: 1896821
EC Number: 200-714-4
MDL number: MFCD00007140
PubChem Substance ID: 24894189
NACRES: NA.26 |
|
PROPERTIES
product line BioReagent
Quality Level 200
Assay ≥98%
form powder
greener alternative product characteristics
Atom Economy
Design for Energy Efficiency
Use of Renewable Feedstocks
Learn more about the Principles of Green Chemistry.
mp 240-245 °C (dec.) (lit.)
suitability suitable for determination of sulfhydryl groups
greener alternative category Re-engineered
SMILES string OC(=O)c1cc(SSc2ccc(c(c2)C(O)=O)[N+]([O-])=O)ccc1[N+]([O-])=O
InChI 1S/C14H8N2O8S2/c17-13(18)9-5-7(1-3-11(9)15(21)22)25-26-8-2-4-12(16(23)24)10(6-8)14(19)20/h1-6H,(H,17,18)(H,19,20)
InChI key KIUMMUBSPKGMOY-UHFFFAOYSA-N
DESCRIPTION
General description
5,5′-Dithiobis(2-nitrobenzoic acid) is also called DTNB or Ellman′s reagent.[1] It is a water soluble reagent, which interacts with thiol groups, and can be used conveniently at neutral pH.[2] Thiolate anion (RS-) reacts with Ellman′s reagent (DTNB2-), to produce 2-nitro-5-thiobenzoate anion (TNB2-) and one mixed disulfide (R-S-TNB-). TNB2- has a high molar absorption coefficient, and absorbs at 412nm. This absorption is independent of pH, at pH> 7.3.[3]
Application
Ellman′s reagent maybe used for acetylcholinesterase assay, and to specifically label cysteine residues.[2] It is a sensitive reagent for measuring the free sulfhydryl content in proteins, peptides, and tissues. |
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