Sigma Aldrich E9644 hEGF EGF, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture 5 x 0.2 mg
Marka
Stok Kodu
LB.SA.E9644-5X.2MG
Kısa Bilgi
Synonym(s): Epidermal Growth Factor human, EGF CAS Number: 62253-63-8 MDL number: MFCD00132565 NACRES: NA.77
| Sigma Aldrich E9644 Epidermal Growth Factor, human |
|
Synonym(s): Epidermal Growth Factor human, EGF
CAS Number: 62253-63-8
MDL number: MFCD00132565
NACRES: NA.77 |
|
PROPERTIES
biological source human
Quality Level 200
recombinant expressed in E. coli
Assay ≥97% (SDS-PAGE)
form lyophilized powder
potency 0.05-0.6 ng/mL
mol wt 16.0 kDa
packaging pkg of 4X25 μg pkg of 25 μg
storage condition avoid repeated freeze/thaw cycles
technique(s) cell culture | mammalian: suitable
impurities ≤1.00 EU/μg
color white to faint yellow cast
solubility water: soluble 0.025 mg, clear, colorless to faintly yellow
UniProt accession no. P09038
storage temp. −20°C
Gene Information human ... FGF2 (2257)
DESCRIPTION
General description
Fibroblast growth factor-basic (FGF-Basic) is found in basement membranes and sub-endothelial extracellular matrix. FGF-Basic is induced early in the development of the embryo where it is necessary (but not sufficient) for pluripotency and self-renewal of embryonic stem cells (ESC) and lineage defined stem cells; wherein it functions to support proliferation and inhibit differentiation. Self-renewal of hESC depends upon activation of the Activin/Nodal/Smad2,3 branch and suppression of the BMP/GDF/Smad5 branch of the TGFβ-family cell signaling program. FGF-Basic is a competence factor for Nodal support of pluripotency. Activin-A is necessary and sufficient to maintain stemness. It induces the expression of both Nodal and FGF-Basic in human ES cells.
Application
Fibroblast growth factor-basic (FGF-Basic) is required for the maintenance of human embryonic stem cells in culture, and various other stem cell lines, such as mesenchymal stem (stroma) cells (MSC).[1][2][3]
Physical form
Lyophilized from 0.2 μm filtered solution in 20 mM Tris and 1 M NaCl, pH 7.0, containing 50 μg bovine serum albumin per 1 μg BFGF.
Analysis Note
The bioactivity of FGF-Basic was measured in a fluorometric assay using the redox sensitive dye, Resazurin. |
