Sigma-Aldrich F2442 Fetal Bovine Serum USA origin, sterile-filtered, suitable for cell culture, suitable for hybridoma 500 mL
Marka
Stok Kodu
LB.SA.F2442-500ML
| Sigma-Aldrich F2442 Fetal Bovine Serum USA origin, sterile-filtered, suitable for cell culture, suitable for hybridoma 500 mL |
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Synonym(s): Fetal Bovine Sera, Fetal Calf Serum, FBS, FCS, sera, serum
MDL number: MFCD00132239
eCl@ss: 32160409
NACRES: NA.71
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PROPERTIES
biological source bovine fetus
Quality Level 500
sterility sterile-filtered
product line FBS Classic
composition
Bovine IgG, ≤1 mg/mL
Hemoglobin, ≤20 mg/dL
origin USA origin
technique(s)
cell culture | hybridoma: suitable
cell culture | mammalian: suitable
impurities ≤10 EU/mL endotoxin
shipped in dry ice
storage temp. −20 °C
DESCRIPTION
General description
Fetal Bovine Serum (FBS) is a complex mixture with low and high molecular weight biomolecules exhibiting optimal growth-enhancing and growth-suppressing functions. FBS contains molecules, such as growth factors, proteins, vitamins, trace elements, hormones etc. These molecules are important for the growth and maintenance of cells. It is a common supplement for cell culture media. FBS is derived from the calf blood. It is devoid of fibrin and clotting factors. Heat inactivation of FBS blocks the complement system. FBS derived from clotted blood is the most widely used undefined supplement in eukaryotic, especially mammalian, cell culture. It is typically used with the classical media that were developed in the 1950s and 1960s. These include many popular media such as Minimum Essential Medium (MEM), Dulbecco′s Modified Eagles Media (DME, DMEM); Iscove′s Modified Eagles Medium (IMDM), M199 and Roswell Park Memorial Institute medium, RPMI-1640.
Application
Fetal Bovine Serum has also been used as a component of:
perivascular-resident macrophage-like melanocytes (PVM/M) growth medium
synthetic oviduct fluid (SOF)
Eagle′s minimum essential medium (EMEM)
neurobasal medium
Hyclone McCoys 5 A medium
Hanks medium
Biochem/physiol Actions
FBS remains a popular media supplement because it provides a wide array of functions in cell culture. FBS delivers nutrients, growth and attachment factors and protects cells from oxidative damage and apoptosis by mechanisms that are difficult to reproduce in serum-free media (SFM) systems.
Analysis Note
Endotoxin and hemoglobin tested
Tested for the presence of bacteria, virus, and mycoplasma
Triple filtered with 0.1 micron membrane under aseptic conditions |