Sigma-Aldrich G4505 Guanidine hydrochloride ≥99% (titration), organic base and chaeotropic agent 1 kg
Marka
Stok Kodu
LB.SA.G4505-1KG
| Sigma-Aldrich G4505 Guanidine hydrochloride ≥99% (titration), organic base and chaeotropic agent 1 kg |
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Synonym(s): Aminoformamidine hydrochloride, Aminomethanamidine hydrochloride, Guanidinium chloride
Linear Formula: NH2C(=NH)NH2 · HCl
CAS Number: 50-01-1
Molecular Weight: 95.53
Beilstein: 3591990
EC Number: 200-002-3
MDL number: MFCD00013026
PubChem Substance ID: 24895176
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PROPERTIES
biological source synthetic (organic)
Quality Level 400
assay ≥99% (titration)
form crystalline powder
technique(s) RNA extraction: suitable
impurities ≤0.3% water (Karl Fischer)
color white pH (25 °C, 4.6 - 6 at 573 g/L)
mp 180-185 °C (lit.)
solubility H2O: 6 M
density 1.3 g/cm3 (lit.)
absorption ≤0.10 at 260 nm in H2O at 6 M
SMILES string Cl[H].NC(N)=N InChI 1S/CH5N3.ClH/c2-1(3)4;/h(H5,2,3,4);1H InChI key PJJJBBJSCAKJQF-UHFFFAOYSA-N
Gene Information human... KCNA1(3736), KCNA10(3744), KCNA2(3737), KCNA3(3738), KCNA4(3739), KCNA5(3741), KCNA6(3742), KCNA7(3743), KCNB1(3745), KCNB2(9312), KCNC1(3746), KCNC2(3747), KCNC3(3748), KCNC4(3749), KCND1(3750), KCND2(3751), KCND3(3752), KCNF1(3754), KCNG1(3755), KCNG2(26251), KCNG3(170850), KCNG4(93107), KCNH1(3756), KCNH2(3757), KCNH3(23416), KCNH4(23415), KCNH5(27133), KCNH6(81033), KCNH7(90134), KCNH8(131096), KCNQ1(3784), KCNQ2(3785), KCNQ3(3786), KCNQ4(9132), KCNQ5(56479), KCNS1(3787), KCNS2(3788), KCNS3(3790), KCNV1(27012), KCNV2(169522)
DESCRIPTION
General description
Application
Guanidine hydrochloride (GdnHCl) has been used:
to perform absolute quantitation of amyloid-beta (Aß) isoforms
in plasma in protein carbonylation assay
for the quantification of glycosaminoglycans (GAG)
for denaturation of cow′s milk allergens
as a control in antiviral screening tests with coxsackievirus B3 (CVB3)
in RNA isolation to dissociate nucleoproteins and inhibit RNase
Strong chaotropic agent useful for the denaturation and subsequent refolding of proteins. This strong denaturant can solubilize insoluble or denatured proteins such as inclusion bodies. This can be used as the first step in refolding proteins or enzymes into their active form. Urea and dithiothreitol (DTT) may also be necessary.
Packaging
25, 100, 500 g in poly bottle 1 kg in poly bottle 2, 10, 25 kg in poly drum G4505-1KT: Each kit contains 3 x 100G samples, each sample from a uniquely manufactured lot.
Biochem/physiol Actions
Guanidine hydrochloride (GdnHCl) is hydroscopic and a small molecule. It is a potent denaturant of enzymes, which results in conformational changes in the enzyme, leading to complete loss of enzyme activity. GdnHCl is involved in blocking the activity of heat shock protein 104 (Hsp104) adenosine triphosphatase (ATPase) in vivo. It is involved in denaturing alkaline phosphatase (ALPase) in Haliotis diversicolor. GdnHCl leads to the deactivation of enzymes like papain and aminoacylase. GdnHCl, at higher concentrations, is effective in virus inactivation, such as herpes simplex virus 1 (HSV-1).
Caution
May agglomerate upon storage. The quality of the product does not appear to be affected and solutions prepared from the free-flowing and lumpy guanidine hydrochloride appear identical. For free-flowing material, please see our Redi-Dri™ free-flowing product number RDD001.
Preparation
Note In order to make an 8M solution in water, one must heat the solution to 35 °C for approximately 30 minutes. The maximum solubility of guanidine hydrochloride in water at room temperature is approximately 6M. Legal
Information
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