Sigma-Aldrich G5516 Glycerol for molecular biology, ≥99.0% 500 mL
Marka
Stok Kodu
LB.SA.G5516-500ML
Kısa Bilgi
Synonym(s): 1,2,3-Propanetriol, Glycerin Linear Formula: HOCH2CH(OH)CH2OH CAS Number: 56-81-5 Molecular Weight: 92.09 Beilstein: 635685 EC Number: 200-289-5 MDL number: MFCD00004722 PubChem Substance ID: 24895216 NACRES: NA.31
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Sigma-Aldrich G5516 Glycerol for molecular biology, ≥99.0% 500 mL
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Synonym(s): 1,2,3-Propanetriol, Glycerin
Linear Formula: HOCH2CH(OH)CH2OH
CAS Number: 56-81-5
Molecular Weight: 92.09
Beilstein: 635685
EC Number: 200-289-5
MDL number: MFCD00004722
PubChem Substance ID: 24895216
NACRES: NA.31
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PROPERTIES
grade for molecular biology, reagent
Quality Level 200
vapor density 3.1 (vs air)
vapor pressure <1 mmHg ( 20 °C)
Assay ≥99.0%
form liquid
autoignition temp. 698 °F
technique(s) MALDI-MS: suitable
refractive index n20/D 1.474 (lit.)
pH 5.5-8
bp 182 °C/20 mmHg (lit.)
mp 20 °C (lit.)
density 1.25 g/mL (lit.)
cation traces
Fe: ≤5 ppm
Mg: ≤5 ppm
heavy metals (as Pb): <5 ppm
foreign activity DNase, RNase, NICKase, and protease, none detected
SMILES string OCC(O)CO
InChI 1S/C3H8O3/c4-1-3(6)2-5/h3-6H,1-2H2
InChI key PEDCQBHIVMGVHV-UHFFFAOYSA-N
DESCRIPTION
General description
Glycerol is odourless, colorless, viscous in nature, and exists as a sweet tasting liquid. It can be derived naturally as well as from petrochemical feedstock. Glycerol has a wide variety of applications, and is one of the most valuable and versatile chemical substances in nature. It can be used as an emollient, solvent, sweetening agent, in pharmaceutical formulations, cosmetics, foodstuffs and toiletries. It is very stable and can be easily stored under normal temperature; also it is non-irritating and has no adverse impact to the environment.[1][2]
Application
Glycerol has been used as:
- a component of mounting medium for immunofluorescence
- a supplement during cell culture of Mycobacterium tuberculosis and Mycobacterium avium.[2]
- a fuel during the designing of enzymatic biofuel cells.[3]
- a liquid composite matrix with 4-HCCA and 3-aminoquinoline for analysis of neutral and acidic glycans.[4][5]
- a matrix for fast atom bombardment MS.[4][5]
Glycerol may be employed as a liquid matrix for the quantification studies by MALDI (Matrix-assisted laser desorption/ionization mass spectrometry) analysis.[6]
Glycerol is used both in sample preparation and gel formation for polyacrylamide gel electrophoresis. Glycerol (5-10%) increases the density of a sample so that the sample will layer at the bottom of a gel′s sample well. Glycerol is also used to aid in casting gradient gels and as a protein stabilizer and storage buffer component.
Biochem/physiol Actions
Glycerol is hygroscopic in nature and is soluble in water owing to its three hydrophilic alcoholic hydroxyl groups. It can form both inter- and intramolecular hydrogen bonds, making it a very flexible molecule. The physiologic effect of glycerine is due to cell-mediated immunity, increased IgG production and increased histamine release.[1][2]
Features and Benefits
This product has been analyzed for the presence of nucleases and proteases.
Other Notes
The product is intended for laboratory use only, and it is not suitable for drug, household, or other purposes.
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