Sigma-Aldrich G7017 β-Glucuronidase from Helix pomatia Type HP-2, aqueous solution, ≥100,000 units/mL 1 mL
Marka
Stok Kodu
LB.SA.G7017-1ML
Kısa Bilgi
Synonym(s): β-D-Glucuronide glucuronosohydrolase CAS Number: 9001-45-0 Enzyme Commission number: 3.2.1.31 (BRENDA, IUBMB) MDL number: MFCD00130629 NACRES: NA.54
| Sigma-Aldrich G7017 β-Glucuronidase from Helix pomatia |
|
Synonym(s): β-D-Glucuronide glucuronosohydrolase
CAS Number: 9001-45-0
Enzyme Commission number: 3.2.1.31 (BRENDA, IUBMB)
MDL number: MFCD00130629
NACRES: NA.54 |
|
PROPERTIES
biological source Helix pomatia
Quality Level 300
type Type HP-2
form aqueous solution
specific activity ≥100,000 units/mL
secondary activity ≤7,500 units/mL sulfatase
shipped in wet ice
storage temp. 2-8°C
DESCRIPTION
General description
β-Glucuronidase Type H-2 from Helix pomatia is a crude solution of enzymes derived from the digestive juices of the Roman snail. β-glucuronidases derived from mollusks often contain sulfatase activity. Both activities are useful in the hydrolysis of metabolites for toxicology and biomarker analysis. For a broader look at our β-Glucuronidase product line and their uses see our page about Drug Metabolite Analysis
Application
Sample preparation for LC/MS through digestion of glucuronide conjugated compounds for biomarker and toxicology research. Removal of glucuronide conjugates such as for the preparation of Baicalein.
New Technical Article Comparing Performance of Different Enzymes
Learn more
about recent application data generated by Sigma R&D to optimize hydrolysis for different drug classes using enzymes from different sources and the use of a chromatographicaly purified enzyme to reduce the effect of esterase activity resulting in conversion of 6-MAM to Morphine
Packaging
1, 2, 5, 10, 25, 100 mL
Biochem/physiol Actions
Endogenous beta-glucuronidase is a lysosomal enzyme involved in digestion of glucuronide-modified catabolites. Microbial beta-glucuronidase found in microbiota can cause re-absorption of metabolites by hydrolysis of glucuronide conjugates of xenobiotic and endobiotic metabolites.[1] Used for the hydrolysis of glucuronide conjugates in urinary metabolite analysis
Features and Benefits
pH optima 4.0 - 5.0.[2]
Sulfatase pH optima 6.2
Low Sulfatase activity (<7,500 units/mL).
Ammonium Sulfate suspension.
Mollusk-derived beta-glucuronidase.
Useful for acidic hydrolysis of glucuronides in low pH samples.
Quality
Many β-glucuronidases derived from mollusks also contain sulfatase activity.
Linkage
Similar to G0876, but produced by Sigma.
Unit Definition
One Sigma or modified Fishman unit will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide per hr at 37 °C at pH 5.0 (30 min assay). Sulfatase Unit Definition: One unit of sulfatase will hydrolyze 1.0 μmole p-nitrocatechol sulfate per hr at pH 5.0 at 37 °C.
Physical form
Aqueous solution in ~1.0 M ammonium sulfate with 3 mM sodium azide as preservative. |
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