Sigma-Aldrich G9277 Guanidine thiocyanate for molecular biology, ≥99% 500 gr
Marka
Stok Kodu
LB.SA.G9277-500G
Kısa Bilgi
Synonym(s): Guanidinium rhodanide, Guanidinium thiocyanate Linear Formula: NH2C(=NH)NH2 · HSCN CAS Number: 593-84-0 Molecular Weight: 118.16 Beilstein: 3563461 EC Number: 209-812-1 MDL number: MFCD00013027 PubChem Substance ID: 24895367 NACRES: NA.31
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Sigma-Aldrich G9277 Guanidine thiocyanate for molecular biology, ≥99% 500 gr |
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Synonym(s): Guanidinium rhodanide, Guanidinium thiocyanate
Linear Formula: NH2C(=NH)NH2 · HSCN
CAS Number: 593-84-0
Molecular Weight: 118.16
Beilstein: 3563461
EC Number: 209-812-1
MDL number: MFCD00013027
PubChem Substance ID: 24895367
NACRES: NA.31 |
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PROPERTIES
biological source synthetic (organic)
Quality Level 200
grade for molecular biology
Assay ≥99%
form powder or crystals
technique(s) RNA extraction: suitable
color colorless to white
mp 120-122 °C (lit.)
solubility water: 100 mg/mL, clear, colorless
cation traces Ba: ≤5 ppm, Fe: ≤5 ppm, Na: ≤0.5%, heavy metals (as Pb): ≤5 ppm
application(s) agriculture
foreign activity DNase, RNase, none detected
SMILES string SC#N.NC(N)=N
InChI 1S/CH5N3.CHNS/c2-1(3)4;2-1-3/h(H5,2,3,4);3H
InChI key ZJYYHGLJYGJLLN-UHFFFAOYSA-N
DESCRIPTION
Application
Guanidine thiocyanate has been used: for the lysis of colorectal adenocarcinoma cell line prior to RNA isolation[1] as a PCR inhibitor in triplex mitochondrial DNA (mtDNA) qPCR assay[2] for DNA extraction from cerebrospinal fluid (CSF)[3] Chaotropic agent and strong denaturant; solubilizes cells.
Biochem/physiol Actions
Guanidine thiocyanate is a denaturing agent and is used routinely in RNA isolation. It is used as a storage buffer for whole blood samples.[4] Guanidine thiocyanate inactivates nucleases and is ideal for storing and freezing fecal samples for DNA studies.[5] It is used in combination with phenol−chloroform in RNA extraction.[6] |
Tavsiye Ürünler
1.430,27 TL + KDV
1.716,32 TL
