Sigma-Aldrich H3375 HEPES ≥99.5% (titration) 25 gr
Marka
Stok Kodu
LB.SA.H3375-25G
| Sigma-Aldrich H3375 HEPES ≥99.5% (titration) 25 gr |
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Synonym(s): 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)
Empirical Formula (Hill Notation): C8H18N2O4S
CAS Number: 7365-45-9
Molecular Weight: 238.30
Beilstein: 883043
EC Number: 230-907-9
MDL number: MFCD00006158
PubChem Substance ID: 24895572
NACRES: NA.25
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PROPERTIES
Quality Level 400
assay ≥99.5% (titration)
form crystalline powder
technique(s) cell culture | mammalian: suitable
pH 5.0-6.5 (25 °C, 238 g/L)
useful pH range 6.8-8.2
pKa (25 °C) 7.5
solubility water: 500 mg/mL, clear, colorless
application(s) diagnostic assay manufacturing
SMILES string OCCN1CCN(CC1)CCS(O)(=O)=O
InChI 1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)
InChI key JKMHFZQWWAIEOD-UHFFFAOYSA-N
DESCRIPTION
General description
HEPES has been described as one of the best all-purpose buffers available for biological research.[1] At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.[2]
Application
HEPES has been used:
As a component in Danieau medium, which is used to prevent melanin pigment formation post gastrulation of zebra-fish embryo[3]
As a component of Hanks′ solution used in the degradation testing of Iron[4]
As a component of HEPES medium in the transfection of cell culture with plasmids[5]
Along with RPMI-1640, L-glutamine, FBS, Sodium pyruvate and β-mercaptoethanol for the culturing of rat insulinoma cells[6]
As a supplement in TCM199 medium for washing fresh oocytes required for nuclear transfer studies[7]
Packaging
25, 100, 250, 500 g in poly bottle
1 kg in poly bottle
5, 25, 50 kg in poly drum
Other Notes
Go to Redi-Dri™ Product Listing RDD002 Easily compare specifications for HEPES products with the HEPES specification table.
Legal Information
Redi-Dri is a trademark of Sigma-Aldrich Co. LLC
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