Sigma-Aldrich H4791 Bone Morphogenetic Protein 2 human BMP-2, recombinant, expressed in HEK 293 cells, HumanKine, suitable for cell culture 10 μgr
| Sigma-Aldrich H4791 Bone Morphogenetic Protein 2 human BMP-2, recombinant, expressed in HEK 293 cells, HumanKine, suitable for cell culture 10 μgr |
|
Synonym(s): BMP-2
MDL number: MFCD03097877
NACRES: NA.77
SDS İçin Resmi Tıklayınız... |
| PROPERTIES
biological source human
Quality Level 100
recombinant expressed in HEK 293 cells
form lyophilized powder
potency ≤60 ng/mL EC50
quality endotoxin tested
mol wt dimer 30-38 kDa (glycosylated)
packaging pkg of 10 μg
storage condition avoid repeated freeze/thaw cycles
technique(s) cell culture | mammalian: suitable
impurities ≤1 EU/μg Endotoxin level
UniProt accession no. P12643
storage temp. −20 °C
Gene Information human... BMP2(650)
DESCRIPTION
General description
Bone Morphogenetic Protein 2 is a member of the TGF-β superfamily of cytokines that affect bone and cartilage formation. It is important for skeletal development during embryogenesis. BMP-2 induces chondrocyte formation, osteoblast differentiation, and is involved in embryo dorsal-ventral patterning and organogenesis.
Packaging 10 μg in poly bottle
Biochem/physiol Actions
It has been reported that Bone Morphogenetic Protein 2 (BMP-2) inhibits estradiol induced proliferation of human breast cancer cells. BMP-2 signaling mediates apoptosis by activation of the TAK1-p38 kinase pathway that is negatively regulated by Smad6. Cellular responses to BMP-2 are mediated by the formation of hetero-oligomeric complexes of type I and type II serine/threonine kinase receptors, which play significant roles in BMP binding and signaling. One BMP type II receptor and two BMP type I receptors have been identified. Both BMP type I receptors bind BMP-2 with high-affinity in the absence of BMP receptor type II.
Physical form
Lyophilized from a 0.2 μm filtered solution of 2x PBS + 6% Ethanol.
Preparation Note
This Bone Morphogenetic Protein 2 (BMP-2) is produced from a DNA sequence encoding the human BMP-2 protein, expressed in HEK 293 cells. It is a glycosylated homodimer linked by a single disulfide bond with an apparent molecular mass of 30-38 kDa.
Analysis Note
The specific activity was determined by its ability to induce alkaline phosphatase production in a dose response to BMP-2 in the ATDC-5 cell line (mouse chondrogenic cell line).
Legal Information
HumanKine is a registered trademark of Proteintech Group, Inc. |