Sigma-Aldrich I6758 IPTG ≥99% (TLC), ≤0.1% Dioxane 5 gr
Marka
Stok Kodu
LB.SA.I6758-5G
Kısa Bilgi
Synonym(s): Isopropyl β-D-1-thiogalactopyranoside, IPTG, Isopropyl β-D-thiogalactoside Empirical Formula (Hill Notation): C9H18O5S CAS Number: 367-93-1 Molecular Weight: 238.30 Beilstein: 4631 EC Number: 206-703-0 MDL number: MFCD00063273 PubChem Substance ID: 24896093 NACRES: NA.31
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Sigma-Aldrich I6758 IPTG ≥99% (TLC), ≤0.1% Dioxane 5 gr |
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Synonym(s): Isopropyl β-D-1-thiogalactopyranoside, IPTG, Isopropyl β-D-thiogalactoside
Empirical Formula (Hill Notation): C9H18O5S
CAS Number: 367-93-1
Molecular Weight: 238.30
Beilstein: 4631
EC Number: 206-703-0
MDL number: MFCD00063273
PubChem Substance ID: 24896093
NACRES: NA.31
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PROPERTIES
biological source synthetic
Quality Level 300
description non-ionic
assay ≥99% (TLC)
form powder
technique(s) thin layer chromatography (TLC): suitable
impurities ≤0.1% Dioxane
storage temp. 2-8°C
SMILES string CC(C)S[C@@H]1OC(CO)[C@H](O)C(O)[C@H]1O
InChI 1S/C9H18O5S/c1-4(2)15-9-8(13)7(12)6(11)5(3-10)14-9/h4-13H,3H2,1-2H3/t5-,6+,7+,8-,9+/m1/s1
InChI key BPHPUYQFMNQIOC-NXRLNHOXSA-N
DESCRIPTION
Packaging
1, 5, 10 g in glass bottle
Application
IPTG is commonly used in cloning procedures that require induction of β-galactosidase activity. It is used in conjunction with X-Gal or Bluo-Gal in blue-white selection of recombinant bacterial colonies that induce expression of the lac operon in Escherichia coli. IPTG functions by binding to the lacI repressor and altering its conformation, which prevents the repression of the β-galactosidase coding gene lacZ.
Other Notes
Non-metabolizable galactose analog.
Reconstitution
A stock solution (0.1 M) is prepared by dissolving IPTG in water with subsequent sterile filtration of the solution. The final concentration of IPTG in indicator plates should be 0.2 mM. |