Sigma-Aldrich I9516 2-Propanol BioReagent, for molecular biology, ≥99.5% 1 L
Marka
Stok Kodu
LB.SA.9516-1L
Kısa Bilgi
Synonym(s): sec-Propyl alcohol, IPA, Isopropanol, Isopropyl alcohol Linear Formula: (CH3)2CHOH CAS Number: 67-63-0 Molecular Weight: 60.10 Beilstein: 635639 EC Number: 200-661-7 MDL number: MFCD00011674 PubChem Substance ID: 24896158 NACRES: NA.52
Bilgi
DESCRIPTION
Sigma-Aldrich I9516 2-Propanol BioReagent, for molecular biology, ≥99.5% 1 L |
Synonym(s): sec-Propyl alcohol, IPA, Isopropanol, Isopropyl alcohol
Linear Formula: (CH3)2CHOH
CAS Number: 67-63-0
Molecular Weight: 60.10
Beilstein: 635639
EC Number: 200-661-7
MDL number: MFCD00011674
PubChem Substance ID: 24896158
NACRES: NA.52 |
PROPERTIES
grade for molecular biology
Quality Level 200
vapor density 2.1 (vs air)
vapor pressure 33 mmHg ( 20 °C) 44 mmHg ( 25 °C)
product line BioReagent
assay ≥99.5%
form liquid
autoignition temp. 750 °F
expl. lim. 2.0-12.7 %, 93 °C
technique(s)
DNA extraction: suitable
RNA extraction: suitable
impurities ≤0.2% water water
color colorless
refractive index n20/D 1.377 (lit.)
bp 82 °C (lit.)
mp −89.5 °C (lit.)
solubility water: soluble (completely)
density 0.785 g/mL at 25 °C (lit.)
suitability suitable for precipitation of nucleic acids
storage temp. room temp
SMILES string CC(C)O
InChI 1S/C3H8O/c1-3(2)4/h3-4H,1-2H3
InChI key KFZMGEQAYNKOFK-UHFFFAOYSA-N
DESCRIPTION
General description
Isopropanol is a clear, colorless, polar organic solvent commonly used in chemistry and molecular biology laboratories. It will dissolve a wide range of chemicals, and evaporates quickly. This product (2-propanol) is designated as molecular biology grade and is suitable for the precipitation of nucleic acids.
Protocols for the use of isopropanol:
DNA precipitation from mammalian tissue, ethidium bromide extraction from DNA samples, and RNA isolation from cells and tissues.
Protein crystallization.
Isolate retinoids from biological matrices for HPLC and HPLC-MS analysis.
Application
2-Propanol has been used:
in DNA and RNA extraction
for determination of hydroxyproline levels(The Merck Index, 12th ed)
for checking cell viability by MTT assay
in microarray hybridization
Suitable for use in DNA precipitation using standard protocols. Also suitable as a solvent for making solutions for molecular biology applications.
Packaging
25, 25, 500 mL in glass bottle
1, 4 L |
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