Sigma-Aldrich K1377 Kanamycin sulfate from Streptomyces kanamyceticus powder, BioReagent, suitable for cell culture, suitable for plant cell culture 25 gr
Marka
Stok Kodu
LB.SA.K1377-25G
Kısa Bilgi
Synonym(s): Kanamycin A, Kanamycin sulfate salt Empirical Formula (Hill Notation): C18H36N4O11 · H2O4S CAS Number: 25389-94-0 Molecular Weight: 582.58 EC Number: 246-933-9 MDL number: MFCD00070253 PubChem Substance ID: 24896204 NACRES: NA.76
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Sigma-Aldrich K1377 Kanamycin sulfate from Streptomyces kanamyceticus 25 gr
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Synonym(s): Kanamycin A, Kanamycin sulfate salt
Empirical Formula (Hill Notation): C18H36N4O11 · H2O4S
CAS Number: 25389-94-0
Molecular Weight: 582.58
EC Number: 246-933-9
MDL number: MFCD00070253
PubChem Substance ID: 24896204
NACRES: NA.76
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PROPERTIES
biological source Streptomyces kanamyceticus
Quality Level 200
product line BioReagent
form powder
potency ≥750 μg per mg (dry basis)
technique(s) cell culture | mammalian: suitable cell culture | plant: suitable
impurities ≤5% kanamycin B
color white to off-white
solubility H2O: 10-50 mg/mL (As a stock solution. Stock solutions should be stored at 2-8°C. Stable at 37°C for 5 days.)
antibiotic activity spectrum
Gram-negative bacteria
Gram-positive bacteria mycobacteria mycoplasma
application(s) agriculture
Mode of action protein synthesis | interferes
SMILES string OS(O)(=O)=O.NC[C@H]1O[C@H](O[C@@H]2[C@@H](N)C[C@@H](N)[C@H](O[C@H]3O[C@H](CO)[C@@H](O)[C@H](N)[C@H]3O)[C@H]2O)[C@H](O)[C@@H](O)[C@@H]1O
InChI 1S/C18H36N4O11.H2O4S/c19-2-6-10(25)12(27)13(28)18(30-6)33-16-5(21)1-4(20)15(14(16)29)32-17-11(26)8(22)9(24)7(3-23)31-17;1-5(2,3)4/h4-18,23-29H,1-3,19-22H2;(H2,1,2,3,4)/t4-,5+,6-,7-,8+,9-,10-,11-,12+,13-,14-,15+,16-,17-,18-;/m1./s1
InChI key OOYGSFOGFJDDHP-KMCOLRRFSA-N
DESCRIPTION
General description
Chemical structure: aminoglycoside
Kanamycin sulfate is a broad spectrum aminoglycoside-antibiotic derived from Streptomyces kanamyceticus. It is used as an additive in culture media for the isolation of group D streptococci on Kanamycin Esculin Azide Agar and for selection of transformed plant cells containing the neomycin phosphotransferase on a kanamycin-medium. Kanamycin sulfate can also be used as a selection agent for cells transformed with kanamycin B resistance gene. It is recommended for use in cell culture applications at 100μg/mL.
Application
Kanamycin sulfate from Streptomyces kanamyceticus has been used in tissue culture media.[1]
Packaging
1, 5, 25 g in poly bottle
Biochem/physiol Actions
Mode of Action: The product acts by binding to the 70S ribosomal subunit, inhibiting translocation and eliciting miscoding.
Mode of Resistance:Aminoglycoside-modifying enzymes (including acetyltransferase, phosphotransferase, nucleotidyltransferase) can alter this antibiotic, preventing its interaction with ribosomes.
Antimicrobial spectrum: Kanamycin sulfate is effective against gram-negative and gram-postiive bacteria, and mycoplasma.
Caution Solutions are stable at 37°C for approximately 5 days. Aqueous stock solutions can be stored at 2-8°C for long term storage.
Preparation Note
Kanamycin sulfate is soluble in water at 50 mg/mL, yielding a clear solution. It is practically insoluble in alcohol, acetone, chloroform, ether and ethyl acetate. A 1% solution in water has a pH of 6.5 to 8.5. Sterile solutions can be prepared by a sterile filtration, through a .2μm filter.
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