Sigma-Aldrich KCQS04 KiCqStart ® Probu qPCR ReadyMix ™ for Bio-Rad, Cepheid, Eppendorf, Illumina, Corbett, and Roche systems 250 Reactions
Marka
Stok Kodu
LB.SA.KCQS04-250RXN
Kısa Bilgi
Synonym(s): Kicqstart® Probe qPCR ReadyMix™ UNSPSC Code: 41106300 NACRES: NA.55
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Sigma-Aldrich KCQS04 KiCqStart® Probu qPCR ReadyMix™
|
| Synonym(s): Kicqstart® Probe qPCR ReadyMix™ UNSPSC Code: 41106300 NACRES: NA.55 |
|
PROPERTIES
form liquid
usage sufficient for 1250 reactions sufficient for 250 reactions sufficient for 5000 reactions feature dNTPs included hotstart storage condition protect from light technique(s) qPCR: suitable color light blue input purified DNA compatibility Bio-Rad CFX384 Bio-Rad CFX96 Bio-Rad MJ Chromo4 Bio-Rad MJ Opticon 2 Bio-Rad MJ Opticon Bio-Rad MiniOpticon Cepheid SmartCycler Eppendorf® Mastercycler ep realplex Eppendorf® Mastercycler ep realplex2 S Illumina Eco qPCR Qiagen Corbett Rotor-Gene 3000 Qiagen Corbett Rotor-Gene 6000 Qiagen Corbett Rotor-Gene Q Roche LightCycler 480 detection method probe-based shipped in dry ice DESCRIPTION General description KiCqStart® Probe qPCR ReadyMix™ is an advanced qPCR reagent system for both fast and conventional PCR cycling protocols or instruments. It is a versatile and robust solution that provides the ultimate sensitivity and high PCR efficiency using a variety of fluorogenic probe chemistries, including TaqMan® hydrolysis probes. KiCqStart Probe qPCR ReadyMix is provided as a 2X concentrated ready-to-use reaction cocktail that contains all required reaction components, except primers, probe(s), and DNA template. The light blue color of an inert tracer dye simplifies reaction assembly in white, or clear, plates and helps to minimize pipetting or mixing errors. It does not interfere with qPCR performance or affect the stability of the product. A key component of KiCqStart Probe qPCR ReadyMix is an ultra-pure, processive thermostable DNA polymerase that is free of detectable E. coli DNA§. This PCR master mix is ideal for demanding qPCR applications such as bacterial pathogen detection where residual host DNA in typical recombinant enzyme preparations can limit assay sensitivity and obscure detection of low copy samples. The enzyme in KiCqStart Probe qPCR ReadyMix is combined with high avidity monoclonal antibodies to provide a stringent automatic hot-start that allows reaction assembly, and temporary storage, at room temperature prior to PCR amplification. Application Different real-time PCR systems employ different strategies for normalization of fluorescent signals and correction of well-to-well optical variations. It is critical to match the appropriate qPCR reagent to your specific instrument.KiCqStart One-Step Probe RT-qPCR ReadyMix does not contain an internal reference dye. Features and Benefits - Assay results in as little as 33 minutes - Highly efficient and sensitive real-time PCR results - Little/no optimization required Components 2X reaction buffer containing optimized concentrations of MgCl2, dNTPs (dATP, dCTP, dGTP, dTTP), hot-start DNA polymerase, insert blue qPCR dye, and stabilizers. packaging: 250 reactions* = 2 X 1.25 mL tubes 1250 reactions* = 10 X 1.25 mL tubes 5000 reactions* = 1 X 50 mL tube *number of reactions based on a 20uL volume Legal Information Eppendorf is a registered trademark of Eppendorf AG KiCqStart is a registered trademark of QIAGEN Beverly Inc. ReadyMix is a trademark of Sigma-Aldrich Co. LLC TaqMan is a registered trademark of Roche Molecular Systems, Inc. |
