Sigma-Aldrich L3126 Lipase from porcine pancreas Type II, ≥125 units/mg protein (using olive oil (30 min incubation)), 30-90 units/mg protein (using triacetin) 25 gr
Marka
Stok Kodu
LB.SA.L3126-25G
Kısa Bilgi
Synonym(s): Pancreatic lipase, PPL, Triacylglycerol acylhydrolase, Triacylglycerol lipase CAS Number: 9001-62-1 Enzyme Commission number: 3.1.1.3 (BRENDA, IUBMB) EC Number: 232-619-9 MDL number: MFCD00131509 NACRES: NA.54
Garanti Süresi
24 Ay
| Sigma-Aldrich L3126 Lipase from porcine pancreas Type II 25 gr |
|
Synonym(s): Pancreatic lipase, PPL, Triacylglycerol acylhydrolase, Triacylglycerol lipase
CAS Number: 9001-62-1
Enzyme Commission number: 3.1.1.3 (BRENDA, IUBMB)
EC Number: 232-619-9
MDL number: MFCD00131509
NACRES: NA.54 |
|
PROPERTIES
biological source Porcine pancreas
Quality Level 200
type Type II
form lyophilized (cake)
specific activity
≥125 units/mg protein (using olive oil (30 min incubation))
30-90 units/mg protein (using triacetin)
composition protein, ≥20%
storage temp. 2-8°C
InChI 1S/C11H9N3O2.Na/c15-8-4-5-9(10(16)7-8)13-14-11-3-1-2-6-12-11;/h1-7,16H,(H,12,14);/q;+1/b13-9-;
InChI key QWZUIMCIEOCSJF-CHHCPSLASA-N
DESCRIPTION
Application
Lipase from porcine pancreas has been used:
for the analysis of fatty acid at the 2-position of triglycerides.[1]
to determine the indigestible fraction of plant source that is resistant to the action of digestive enzymes.[2]
as a component of dissolution media to study the dissolution profiles of theophylline.[3]
Lipases are used industrially for the resolution of chiral compounds and the transesterification production of biodiesel.
Biochem/physiol Actions
Lipases catalyze the hydrolysis of esters in aqueous solutions and the synthesis of esters in non-aqueous solutions. They also produce hydroxyl and carboxylic groups through the hydrolysis of ester linkages in poly(ethylene terephthalate). Lipases catalyze the hydrolysis of triacylglycerols into glycerol and free fatty acids.
Quality
Crude (Steapsin). Contains amylase and protease activity.
Unit Definition
One unit will hydrolyze 1.0 microequivalent of fatty acid from triacetin in 1 hr at pH 7.4 at 37°C. (pH 7.7 is used with olive oil as substrate)
Analysis Note
Protein determined by biuret. |
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