Sigma Aldrich M2128 Thiazolyl Blue Tetrazolium Bromide 98% 5 gr
Marka
Stok Kodu
LB.SA.M2128-5G
Kısa Bilgi
Synonym(s): 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide, MTT, Methylthiazolyldiphenyl-tetrazolium bromide Empirical Formula (Hill Notation): C18H16BrN5S CAS Number: 298-93-1 Molecular Weight: 414.32 Beilstein: 4081397 EC Number: 206-069-5 MDL number: MFCD00011964 PubChem Substance ID: 24896603 NACRES: NA.47
| Sigma Aldrich M2128 Thiazolyl Blue Tetrazolium Bromide 98% 5 gr |
|
Synonym(s): 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide, MTT, Methylthiazolyldiphenyl-tetrazolium bromide
Empirical Formula (Hill Notation): C18H16BrN5S
CAS Number: 298-93-1
Molecular Weight: 414.32
Beilstein: 4081397
EC Number: 206-069-5
MDL number: MFCD00011964
PubChem Substance ID: 24896603
NACRES: NA.47 |
|
PROPERTIES
Quality Level 200
Assay 98%
form powder
mp 195 °C (dec.) (lit.)
solubility
2-methoxyethanol: soluble 20 mg/mL
ethanol: soluble 20 mg/mL
H2O: 5 mg/mL
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp. 2-8°C
SMILES string [Br-].Cc1nc(sc1C)-[n+]2nc(nn2-c3ccccc3)-c4ccccc4
InChI 1S/C18H16N5S.BrH/c1-13-14(2)24-18(19-13)23-21-17(15-9-5-3-6-10-15)20-22(23)16-11-7-4-8-12-16;/h3-12H,1-2H3;1H/q+1;/p-1
InChI key AZKSAVLVSZKNRD-UHFFFAOYSA-M
DESCRIPTION
Application
Thiazolyl Blue Tetrazolium Blue (MTT) may be used in measurement of cell proliferation. MTT produces a yellowish solution that is converted to dark blue, water-insoluble MTT formazan by mitochondrial dehydrogenases of living cells. The blue crystals are solubilized with acidified isopropanol and the intensity is measured colorimetrically at 570 nm. MTT has been used as a histochemical/cytochemical reagent and for the detection of NAD. ADP-linked enzyme systems in tissue cannot be detected with MTT, due to binding of the cation by the cyanide trap used. MTT is rapidly reduced to the formazan, which chelates with nickel, copper, and cobalt; the cobalt chelate has been used in oxidative systems.
Thiazolyl Blue Tetrazolium Bromide has been used for the following assays:
- Proliferation assay.[1][2]
- TNF (tumor necrosis factor) bioassay.[3] |
Tavsiye Ürünler
