Sigma-Aldrich M4655 Minimum Essential Medium Eagle With Earle′s salts, L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture 1 L
Marka
Stok Kodu
LB.SA.M4655-1L
Kısa Bilgi
Synonym(s): MEM NACRES: NA.75
| Sigma-Aldrich M4655 Minimum Essential Medium Eagle 1 L |
|
Synonym(s): EMEM, MEM
NACRES: NA.75 |
|
PROPERTIES
Quality Level 400
sterility sterile-filtered
form liquid
technique(s) cell culture | mammalian: suitable
impurities endotoxin, tested
components
sodium pyruvate: no
phenol red: yes
Earle’s salts (5% CO2): yes
HEPES: no NaHCO3: yes
L-glutamine: yes
shipped in ambient
storage temp. 2-8°C
DESCRIPTION
General description
Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media. Early attempts to cultivate normal mammalian fibroblasts and certain subtypes of HeLa cells revealed they had specific nutritional requirements that could not be met by Eagle′s Basal Medium (BME). Subsequent studies using these and other cells in culture indicated additions to BME could be made to aid growth of a wider variety of fastidious cells.
MEM, which incorporates these modifications, includes higher concentrations of amino acids so the medium more closely approximates the protein composition of cultured mammalian cells. MEM has been used for cultivation of a wide variety of cells grown in monolayers. Optional supplementation of non-essential amino acids to the formulations that incorporate either Hanks′ or Earle′s salts has broadened the usefulness of this medium.
Packaging
500, 500, 500 mL
1, 1, 10 L
Application
Generally used to grow attached cell lines, such as fibroblasts, in the presence of FBS, calf or horse sera. Other Notes
This product lacks L-Ala; L-Asn; L-Glu; Gly; L-Pro; L-Ser. |
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