Sigma-Aldrich M8410 Mineral oil light oil, (neat) 1 L
Kategori
Marka
Stok Kodu
LB.SA.M8410-1L
Kısa Bilgi
CAS Number: 8042-47-5 EC Number: 232-455-8 MDL number: MFCD00131611
Bilgi
CAS Number: 8042-47-5
| Sigma-Aldrich M8410 Mineral oil light oil, (neat) 1 L |
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CAS Number: 8042-47-5
EC Number: 232-455-8
MDL number: MFCD00131611
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PROPERTIES
Quality Level 200
sterility non-sterile
form light oil
storage condition protect from light
refractive index n20/D 1.467 (lit.)
density 0.84 g/mL at 25 °C (lit.)
format neat
InChI key AEOVEGJBKQQFOP-DDVLFWKVSA-L
DESCRIPTION
Application
Mineral oil has been used as an overlay in microdrop embryo cultures.[1][2][3]
Packaging
5 mL in poly bottle
100, 500 mL in glass bottle 1 L in glass bottle
Caution
This product is not sterile. Mineral oil is difficult to sterilize. It can only be sterilized by careful filtration of the warmed oil. It is assumed antibiotics will be added to the cell culture medium. Otherwise, Prod. No. M5310 should be used, as it is sterile filtered.
Analysis Note
This material is tested as an overlay in microdrop embryo cultures. (All embryo tested materials are tested using mouse embryos.) Typically BCF1 x BDF1 hybrids are used. The oil is primarily used to prevent evaporation of the medium, particiularly when performing embryo manipulations.1 The procedure is as follows: 1-cell (BCF1 X BDF1) embryos were cultured in triplicate microdrops of embryo-tested HTF (Human Tubal Fluid) medium supplemented with 0.4% BSA. For a valid assay, at least 70% of 1-cell stage control embryos must develop to the blastocyst stage within 96 hours. For product release, at least 80% of 1-cell stage test embryos must develop to the blastocyst stage within 96 hours with the tested material.
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