Sigma-Aldrich MAK022 Calcium Colorimetric Assay sufficient for 250 colorimetric tests
| Sigma-Aldrich MAK022 Calcium Colorimetric Assay sufficient for 250 colorimetric tests |
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NACRES: NA.84
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| PROPERTIES
usage sufficient for 250 colorimetric tests
detection method colorimetric
storage temp. 2-8 °C
DESCRIPTION
General description
Calcium, the most abundant mineral in the human body, is a crucial intracellular element that is responsible for regulating many physiological and pathological processes. Calcium is found in either the free ion form or in bound complexes, for example the calcium phosphate and calcium carbonate complexes that make up bone tissue. Numerous physiological processes, including muscle contraction, cell adhesion, hormones/ neurotransmitters release, glycogen metabolism, cell proliferation/differentiation, blood clotting, nerve or synapthetic impulse transmission, and structural support of the skeleton are regulated by calcium signaling. Defects in the integrity of cell-specific calcium signaling systems may be associated with certain human diseases.
Application
Calcium Colorimetric Assay kit has been used to measure calcium concentration in hippocampal samples and MC3T3-E1 mouse osteoblast cell line, which were cultured in osteogenic induction medium.
Features and Benefits
Compatible with high-throughput handling systems.
Suitability
Suitable for use with cell and tissue culture supernatants, urine, plasma, serum, fecal material, media, and other biological fluids.
Principle
In this assay, the calcium ion concentration is determined by the chromogenic complex formed between calcium ions and o-cresolphthalein, which is measured at 575 nm and is proportional to the concentration of calcium ions present. The linear range of detection for this kit is between 0.4-2.0 μg. |