Sigma-Aldrich MAK087 Acid Phosphatase Activity Fluorometric Assay Kit sufficient for 500 fluorometric tests
| Sigma-Aldrich MAK087 Acid Phosphatase Activity Fluorometric Assay Kit |
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EC Number: 231-834-5
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| PROPERTIES
usage sufficient for 500 fluorometric tests
detection method fluorometric
storage temp. −20 °C
Gene Information
human... ACP1(52), ACP2(53) mouse... ACP1(11431), ACP2(11432) rat... ACP1(24161), ACP2(24162)
DESCRIPTION
General description
Acid phosphatases (AP) are a family of enzymes that non-specifically catalyze the hydrolysis of monoesters and anhydrides of phosphoric acid to produce inorganic phosphate at an optimal pH of 4-7. Changes in the levels and activities of AP occur in many pathophysiological conditions including prostate cancer and bone disease.
Features and Benefits
Compatible with high-throughput handling systems.
Suitability
Suitable for the detection of acid phosphatase in serum and other biological samples.
Principle
The AP Activity Fluorometric Assay Kit provides a simple and direct procedure for measuring AP activity in serum and other samples. AP activity is determined by the conversion of the non-fluorescent 4-methylumbelliferyl phosphate disodium salt (MUP) to the fluorescent product 4-methylumbelliferone (4-MU) (λex = 360/λem = 440 nm), proportional to the AP activity present. One unit of AP is the amount of enzyme that will hydrolyze 1.0 μmole of MUP per minute at 25 °C. |