Sigma-Aldrich MAK411 Alkaline Phosphatase Activity Fluorometric Assay Kit sufficient for 500 fluorometric tests
Sigma-Aldrich MAK411 Alkaline Phosphatase Activity Fluorometric Assay Kit |
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PROPERTIES
Quality Level 100
detection method fluorometric
storage temp. −20 °C
DESCRIPTION
General description
Alkaline phosphatase (ALP) catalyzes the hydrolysis of phosphate esters in alkaline buffer and produces an organic radical and inorganic phosphate. The change in alkaline phosphatase level and activity is associated with several diseases in the liver and bones. Alkaline phosphatase is also a popular enzyme conjugated to secondary antibody in ELISA assays.
Features and Benefits
Alkaline Phosphatase Activity Fluorometric Assay Kit:
Compatible with high-throughput handling systems. Simple procedure with results in ∼1 hour.
Ultra-sensitive, with a detection sensitivity of ∼1 μU.
Suitability
The kit is suitable for the measurement of alkaline phosphatase activity in serum, plasma, urine, semen, cell culture media, cells and tissue.
Principle
In the Alkaline Phosphatase Activity Fluorometric Assay Kit, ALP cleaves the phosphate group of the non-fluorescent 4-Methylumbelliferyl phosphate disodium salt (MUP) substrate resulting in an intense fluorescent signal (λEx = 360 nm/λEm = 440 nm). |