Sigma-Aldrich MAK446 Acid Phosphatase Assay Kit Sufficient for 100 colorimetric tests
| Sigma-Aldrich MAK446 Acid Phosphatase Assay Kit Sufficient for 100 colorimetric tests |
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| PROPERTIES
Quality Level 100
detection method colorimetric
relevant disease(s) cancer, prostate tumor; gastrointestinal diseases
storage temp. −20 °C
DESCRIPTION
General description
Acid Phosphatase (ACP) is an enzyme which catalyzes the cleavage of phosphate groups from other molecules during digestion. Acid phosphatase can be found in lysosomes and become active after fusing with endosomes, acidifying the pH and, thus, creating an optimal environment for ACP. ACP can also be found in bone, spleen, liver, kidney and blood. Serum levels can be used as a biomarker for prostatic carcinoma, although prostate-specific antigen (PSA) is more widely used.
Features and Benefits
Linear detection range (20 μL sample): 0.05 to 60 U/L for a 30 minute reaction.
Compatible with high-throughput handling systems.
Suitability
The kit is suitable for the determination of acid phosphatase in biological samples (e.g. plasma, serum, cell lysate, tissue samples.)
Principle
The non-radioactive, colorimetric Acid Phosphatase Assay Kit is based onthe cleavage of p-nitrophenol from the synthetic substrate. p-Nitrophenol becomes intensely colored after addition of the stop reagent. The increase in absorbance at A405 nm after addition of the stop reagent is directly proportional to the enzyme activity. |