Sigma-Aldrich MAK447 Alkaline Phosphatase Assay Kit Sufficient for 250 colorimetric tests
| Sigma-Aldrich MAK447 Alkaline Phosphatase Assay Kit Sufficient for 250 colorimetric tests |
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EC Number: 245-325-0
eCl@ss: 42010105
NACRES: NA.53
SDS İçin Resmi Tıklayınız... |
| PROPERTIES
Quality Level 100
application(s) pharmaceutical
detection method colorimetric
relevant disease(s) cancer; gastrointestinal diseases
storage temp. −20 °C
DESCRIPTION
General description
Alkaline phosphatase (ALP) catalyzes the hydrolysis of phosphate esters in an alkaline environment, resulting in the formation of an organic radical and inorganic phosphate. In mammals, this enzyme is found mainly in the liver and bones. Marked increase in serum ALP levels, a disease known as hyperalkalinephosphatasemia, has been associated with malignant biliary obstruction, primary biliary cirrhosis, primary sclerosing cholangitis, hepatic lymphoma and sarcoidosis.
Features and Benefits
High sensitivity and wide linear range. The detection limit is 2 U/L, linear up to 800 U/L.
Simple "mix-and-measure" procedure allows reliable quantitation of ALP activity within 5 minutes.
Compatible with high-throughput handling systems.
Suitability
The kit is suitable for the quantitative determination of alkaline phosphatase (ALP) activity in serum, plasma etc. Principle
The Alkaline Phosphatase Assay Kit is designed to measure ALP activity directly in biological samples without pretreatment. The improved method utilizes p-nitrophenyl phosphate that is hydrolyzed by ALP into a yellow colored product (maximal absorbance at A405 nm). The rate of the reaction isdirectly proportional to the enzyme activity. |